| Characterization of protein in old myocardial infarction by FTIR micro-spectroscopy. | |
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MedLine Citation:
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PMID: 20714887 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The aim of the present study was to assess whether Fourier transform infrared spectrometry (FTIR) micro-spectroscopy could produce distinct spectral information on protein of old myocardial infarction (OMI) and to set them as molecular markers to diagnose atypical OMI. Paraffin-embedded heart samples were derived from victims dying of OMI. In combination with histological stain, FTIR and infrared micro-spectroscopy, the characteristics of OMI were analyzed morphologically and molecularly. The most relevant bands identified were the amide A, B, I and, II showing crucial spectral differences between apparent normal region and OMI region, including the peak position blue shift and the increased intensity of OMI, moreover relative increase in alpha-helix and decrease in beta-sheet of protein secondary structures in OMI. Comparing to single spectral band, the I1650/I1550 ratio was increased and rationally used as a molecular marker for diagnosing OMI. These novel preliminary findings supported further exploration of FTIR molecular profiling in clinical or forensic study, and were in accordance with histopathology. |
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Authors:
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Na Zheng; Tiantong Yang; Man Liang; Haidong Zhang; Liping Li; Ananda Sunnassee; Liang Liu |
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Publication Detail:
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Type: Journal Article Date: 2010-08-17 |
Journal Detail:
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Title: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban Volume: 30 ISSN: 1672-0733 ISO Abbreviation: J. Huazhong Univ. Sci. Technol. Med. Sci. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101169627 Medline TA: J Huazhong Univ Sci Technolog Med Sci Country: China |
Other Details:
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Languages: eng Pagination: 546-50 Citation Subset: IM |
Affiliation:
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Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. nana96069@163.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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