| Characterization of protein-antiproteinase imbalance in bronchoalveolar lavage from patients with pneumonia. | |
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MedLine Citation:
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PMID: 8143811 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to clarify the mode of inactivation of alpha1-proteinase inhibitor (alpha 1-PI) in pneumonia, 21 immunocompetent patients and 19 immunocompromised patients with acute pneumonia (Groups I and II) were studied. Nine patients successfully treated for pneumonia and 10 healthy volunteers served as controls (Groups III and IV, respectively). The concentrations of alpha 1-PI, elastase and myeloperoxidase (MPO) in bronchoalveolar lavage fluid (BALF) were determined using a luminometric assay. Elastase inhibition capacity was determined using a colorimetric assay. Thus, the functional activity of alpha 1-PI was calculated. Both elastase and MPO were significantly higher in group I, when compared with the other groups. The mean concentration of alpha 1-PI was significantly higher in patients with acute pneumonia (Group I 13 mg.l-1, Group II 4.22 mg.l-1) than in Groups III and IV (2.65 and 0.33 mg.l-1, respectively), whereas, the proportion of active alpha 1-PI was significantly lower in Group I than in the other groups. Only a small proportion was present as a complex with elastase (ca. 5.9%) or in oxidised form (ca. 4.8%), 85% of alpha 1-PI was irreversibly proteolyzed. This resulted in free elastase activity in 7 of the 40 patients (18%) with acute pneumonia. We conclude that functional activity of alpha 1-PI is markedly impaired due to irreversible proteolysis in acute pneumonia, despite high immunological concentrations. |
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Authors:
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J Braun; K Dalhoff; B Schaaf; W G Wood; K J Wiessmann |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology Volume: 7 ISSN: 0903-1936 ISO Abbreviation: Eur. Respir. J. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-04-29 Completed Date: 1994-04-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8803460 Medline TA: Eur Respir J Country: DENMARK |
Other Details:
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Languages: eng Pagination: 127-33 Citation Subset: IM |
Affiliation:
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Clinic of Internal Medicine, Medical University of Lübeck, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Bronchoalveolar Lavage Fluid / chemistry* Female Humans Male Middle Aged Pancreatic Elastase / metabolism Peroxidase / metabolism Pneumonia / metabolism* Proteins / metabolism* alpha 1-Antitrypsin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Proteins; 0/alpha 1-Antitrypsin; EC 1.11.1.7/Peroxidase; EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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