Document Detail


Characterization of prostate cancer in needle biopsy by cathepsin B, cell proliferation and DNA ploidy.
MedLine Citation:
PMID:  20392989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Our objective was to determine localization patterns of three distinct groups of biomarkers (cathepsin B, MIB-1 and DNA ploidy) in prostate needle biopsy sections to establish localization similarities (or differences) in biopsy and retropubic prostatectomy specimens (RPs).
MATERIALS AND METHODS: Prostate needle biopsy specimens and matched RPs from 47 patients with cancer were evaluated. Biopsy and RP sections were stained with anti-cathepsin B (CB) and anti-stefin (cystatin) A (SA) and for cell proliferation and DNA ploidy. The ratio of CB to SA in stained cells was calculated for each biopsy cancer and matched benign prostatic hyperplasia (BPH) sample.
RESULTS: The geometric mean of CB to SA was 1.45 in BPH and 2.99 in cancer specimens (p=0.0001). The percentage of S-phase cells and DNA ploidy status in needle biopsy was associated with cancer volume in RP cases (p=0.03).
CONCLUSION: Our study has indicated that the ratio of CB to SA is significantly higher in prostate cancer biopsy specimens than in BPH. The percentage of S-phase cells and DNA ploidy in needle biopsies predicts cancer volume of RPs. We have shown that localization of three distinct biomarkers in biopsies reliably assesses the nature of prostate cancer in biopsy sections.
Authors:
Junqi Qian; David G Bostwick; Kenneth A Iczkowski; Konjit Betre; Michael J Wilson; Chap LE; Akhouri A Sinha
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-15     Completed Date:  2010-05-18     Revised Date:  2014-07-03    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  719-25     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Biopsy, Needle
Cathepsin B / metabolism*
Cell Growth Processes / physiology
Cystatins / metabolism
DNA, Neoplasm / genetics*
Humans
Immunohistochemistry
Male
Middle Aged
Ploidies*
Prostatic Neoplasms / genetics,  metabolism,  pathology*
Ubiquitin-Protein Ligases / metabolism
Grant Support
ID/Acronym/Agency:
CA100203/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cystatins; 0/DNA, Neoplasm; EC 3.4.22.1/CTSB protein, human; EC 3.4.22.1/Cathepsin B; EC 6.3.2.19/MIB1 ligase, human; EC 6.3.2.19/Ubiquitin-Protein Ligases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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