| Characterization and properties of steroid binding protein in Bufo arenarum serum. | |
| | |
MedLine Citation:
|
PMID: 7980944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Serum steroid binding properties of mature Bufo arenarum females were studied. Binding data obtained using charcoal adsorption assay and equilibrium dialysis methods indicates a single protein, named Bufo arenarum sex binding protein (Ba SBP), which binds 5 alpha-dihydrotestosterone (DHT), testosterone (T), and estradiol-17 beta (E2) with high affinity (10(-7) M-1 - 10(8) M-1) and fair capacity (10(-6) M). Scatchard plot analysis demonstrated the coexistence of two binding sites. Ba SBP has a sedimentation coefficient of 5.2 S in sucrose gradient centrifugation in low salt and under steady-state conditions. The specificity of this protein, determined by competitive binding experiments, is comparable to human SBP. DHT and T bind with higher affinity than E2. Estriol and estrone competed poorly, while diethylstilbestrol and C21 steroids did not compete. The binding capacity of this protein is under estrogenic control. |
| | |
Authors:
|
S N Fernández; Z C Mansilla-Whitacre; D C Miceli |
Related Documents
:
|
2457134 - A hybrid ligand method for androgen receptor measurement. 12788064 - Implications of a polyglutamine tract in the function of the human androgen receptor. 10784124 - Calretinin is not a marker for subdivisions within the suprachiasmatic nucleus. 4085134 - Augmentation of androgen-receptor binding in vitro: studies in normals and patients wit... 11130004 - Cytotoxicity of an 125i-labelled dna ligand. 16096804 - Interactions in pseudorotoxanes based on crown ether-secondary ammonium motifs. a theor... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular reproduction and development Volume: 38 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 1994 Aug |
Date Detail:
|
Created Date: 1995-01-05 Completed Date: 1995-01-05 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 364-72 Citation Subset: IM |
Affiliation:
|
Instituto Superior de Investigaciones Biológicas, Departamento de Biología del Dessarrollo, Tucumán, Argentina. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Binding Sites Bufo arenarum / blood* Chemistry, Physical Dihydrotestosterone / metabolism Estradiol / metabolism Female Ovariectomy Physicochemical Phenomena Protein Binding Sex Hormone-Binding Globulin / isolation & purification, metabolism* Temperature Testosterone / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Sex Hormone-Binding Globulin; 50-28-2/Estradiol; 521-18-6/Dihydrotestosterone; 58-22-0/Testosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pregnancy-associated endometrial expression of antileukoproteinase gene is correlated with epithelio...
Next Document: Effect of IGF-I on pig oocyte maturation, fertilization, and early embryonic development in vitro, a...