Document Detail

Characterization of cytokine/chemokine profiles of severe acute respiratory syndrome.
MedLine Citation:
PMID:  15657466     Owner:  NLM     Status:  MEDLINE    
RATIONALE: There is currently no optimal treatment or effective drug for severe acute respiratory syndrome (SARS), because the immunopathologic mechanism is poorly understood.
OBJECTIVES: To explore the immune mechanism underlying the pathogenesis of SARS, we studied the expression profile of cytokines/chemokines in the blood and the immunopathology of the lung and lymphoid tissues.
METHODS: Fourteen cytokines/chemokines in the blood of 23 patients with SARS were dynamically screened, using a bead-based multiassay system. Reverse transcription-polymerase chain reaction was performed to amplify mRNA. Histopathology of the lung and lymphoid tissues at autopsy was examined, using methods of immunohistochemistry and double immunofluorescence staining.
MAIN RESULTS: Interferon-inducible protein-10 (IP-10) was markedly elevated in the blood during the early stage of SARS, and remained at a high level until convalescence. Moreover, IP-10 was highly expressed in both lung and lymphoid tissues, where monocyte-macrophage infiltration and depletion of lymphocytes were observed. The levels of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 were concomitantly increased in the blood of the patients with superinfection, and the mRNAs for these cytokines were also increased in lung tissues.
CONCLUSIONS: Induction of IP-10 is a critical event in the initiation of immune-mediated acute lung injury and lymphocyte apoptosis during the development of SARS. Superinfection after the immune injury is the main cause of death. The prompt elevation of interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 is a sign of superinfection, indicating a high risk of death.
Yong Jiang; Jun Xu; Chengzhi Zhou; Zhenguo Wu; Shuqing Zhong; Jinghua Liu; Wei Luo; Tao Chen; Qinghe Qin; Peng Deng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-18
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  171     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-08     Completed Date:  2006-06-19     Revised Date:  2014-07-29    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  850-7     Citation Subset:  AIM; IM    
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MeSH Terms
Apoptosis / immunology
Chemokine CCL2 / blood
Chemokines / blood*,  genetics
Cytokines / blood*,  genetics
Gene Expression / physiology
Lung / immunology,  pathology
Lymphocytes / immunology,  pathology
Lymphoid Tissue / pathology
Microscopy, Fluorescence
Middle Aged
Reference Values
Reverse Transcriptase Polymerase Chain Reaction
SARS Virus / immunology
Severe Acute Respiratory Syndrome / diagnosis,  immunology*,  pathology
Superinfection / immunology,  pathology
Reg. No./Substance:
0/Chemokine CCL2; 0/Chemokines; 0/Cytokines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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