| Characterization of prmt7alpha and beta isozymes from Chinese hamster cells sensitive and resistant to topoisomerase II inhibitors. | |
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MedLine Citation:
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PMID: 17049166 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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By selection of genetic suppressor elements (GSEs) conferring resistance to topoisomerase II inhibitors in Chinese hamster cells (DC-3F), we identified a gene encoding two proteins of 78 and 82 kDa which belong to the protein arginine methyltransferase (PRMT) family. Down-regulation of these enzymes (named PRMT7alpha and beta), either induced by an antisense GSE or as observed in the 9-OH-ellipticine (9-OH-E) resistant mutant DC-3F/9-OH-E, was responsible for cell resistance to various DNA damaging agents. Alternative splicing alterations in the 5'-terminal region and changes of the polyadenylation site of PRMT7 mRNAs were observed in these resistant mutant cells. PRMT7alpha and beta are isoforms of a highly conserved protein containing two copies of a module common to all PRMTs, comprising a Rossmann-fold domain and a beta-barrel domain. The C-terminal repeat appears to be degenerate and catalytically inactive. PRMT7alpha and beta form homo- and hetero-dimers but differ by their sub-cellular localization and in vitro recognize different substrates. PRMT7beta was only observed in Chinese hamster cells while mouse 10T1/2 fibroblasts only contain PRMT7alpha. Surprisingly, in human cells the anti-PRMT7 antibody essentially recognized an approximately 37 kDa peptide, which is not formed during extraction, and a faint band at 78 kDa. Analysis of in vitro and in vivo methylation patterns in cell lines under- or over-expressing PRMT7alpha and beta detected a discrete number of proteins which methylation and/or expression are under the control of these enzymes. |
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Authors:
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Laurent Gros; Axelle Renodon-Cornière; Bruno Robert de Saint Vincent; Marcin Feder; Janusz M Bujnicki; Alain Jacquemin-Sablon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-14 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1760 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-07 Completed Date: 2007-01-09 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1646-56 Citation Subset: IM |
Affiliation:
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CNRS FRE2618, Laboratoire de Pharmacologie des Agents Anticancéreux, Institut Bergonié, 229 Cours de l'Argonne, 33076 Bordeaux, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Cricetinae Cricetulus DNA Topoisomerases, Type II / metabolism Dimerization Enzyme Inhibitors / pharmacology* Hela Cells Humans Isoenzymes / chemistry, genetics, metabolism Methylation Methyltransferases / chemistry, genetics Mice Protein-Arginine N-Methyltransferases / chemistry*, genetics*, metabolism Species Specificity Topoisomerase II Inhibitors* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Isoenzymes; 0/Topoisomerase II Inhibitors; EC 2.1.1.-/Methyltransferases; EC 2.1.1.-/Protein-Arginine N-Methyltransferases; EC 2.1.1.125/PRMT7 protein, human; EC 5.99.1.3/DNA Topoisomerases, Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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