| Characterization of primary human keratinocytes transformed by human papillomavirus type 18. | |
| | |
MedLine Citation:
|
PMID: 2452896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Primary human epithelial cells were cotransfected with pHPV-18 and pSV2neo, and cell strains were generated by selecting in G418. One cell strain (FE-A), which exhibits an extended life span, is currently in its 30th passage. In comparison, control cultures can only be maintained up to the seventh passage. Southern blot analysis revealed the presence of at least one intact, integrated viral genome in these cells. FE-A cells showed altered growth properties, characterized by a change in morphology, and clonal density. Differentiation markers analyzed by Western blotting (immunoblotting), such as cytokeratins and involucrin, indicated that the cells resembled a partially differentiated epithelial population. Increased expression of the 40-kilodalton cytokeratin was observed in FE-A cells, similar to that observed in simian virus 40-immortalized human keratinocytes (M. Steinberg and V. Defendi, J. Cell Physiol. 123:117-125, 1985). FE-A cells were also found to be defective in their response to terminal differentiation stimuli. Calcium and 12-O-tetradecanoyl-phorbol-13-acetate treatment induced normal epithelial cells to differentiate, whereas the human papillomavirus 18 (HPV-18)-containing keratinocytes were resistant to these signals, indicating their partially transformed nature. These cells were not able to induce tumors in nude mice over a period of up to 8 months. A second cell strain, FE-H18L, also generated by transfecting HPV-18, also exhibited an extended life span and similar alterations in morphology. Viral RNA transcribed from the early region of HPV-18 was detected in both cell strains by Northern (RNA) blot analysis. These cell strains should provide a useful model for determining the role of HPV in carcinogenesis. |
| | |
Authors:
|
P Kaur; J K McDougall |
Related Documents
:
|
3713226 - Is non-genic inheritance involved in carcinogenesis? a cytotactic model of transformation. 6164666 - Species specificity of guinea pig and human lymphotoxin colony inhibitory activity. 6270026 - Helper virus-dependent rsv rescue from two lines of hamster cells transformed in vivo b... 16729266 - Scanning electron microscopy investigations on bis(2-ethylhexyl)phthalate treated mycob... 23108506 - Effects of t-2 toxin on testosterone biosynthesis in mouse leydig cells. 2252276 - Growth-related gene expression in type 2 alveolar epithelial cells. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of virology Volume: 62 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1988 Jun |
Date Detail:
|
Created Date: 1988-06-20 Completed Date: 1988-06-20 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1917-24 Citation Subset: IM |
Affiliation:
|
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Calcium / pharmacology Cell Differentiation / drug effects Cell Division Cell Transformation, Viral* Cells, Cultured DNA, Viral / genetics Epithelial Cells Epithelium / microbiology* Gene Expression Regulation Immunologic Techniques Keratins / genetics Male Mice Mice, Nude Neoplasms, Experimental / pathology Papillomaviridae / pathogenicity* Protein Precursors / genetics RNA, Messenger / genetics RNA, Viral / genetics |
| Grant Support | |
ID/Acronym/Agency:
|
CA-42792/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Viral; 0/Protein Precursors; 0/RNA, Messenger; 0/RNA, Viral; 60108-77-2/involucrin; 68238-35-7/Keratins; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Monoclonal antibodies define a domain on herpes simplex virus glycoprotein B involved in virus penet...
Next Document: Expression of herpes simplex virus type 1 glycoprotein D deletion mutants in mammalian cells.