Document Detail

Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines.
MedLine Citation:
PMID:  18629868     Owner:  NLM     Status:  MEDLINE    
Hedgehog (Hh) signaling controls pancreatic development and homeostasis; aberrant Hh signaling is associated with several pancreatic diseases. Here we investigated the link between Hh signaling and primary cilia in the human developing pancreatic ducts and in cultures of human pancreatic duct adenocarcinoma cell lines, PANC-1 and CFPAC-1. We show that the onset of Hh signaling from human embryogenesis to fetal development is associated with accumulation of Hh signaling components Smo and Gli2 in duct primary cilia and a reduction of Gli3 in the duct epithelium. Smo, Ptc, and Gli2 localized to primary cilia of PANC-1 and CFPAC-1 cells, which may maintain high levels of nonstimulated Hh pathway activity. These findings indicate that primary cilia are involved in pancreatic development and postnatal tissue homeostasis.
Sonja K Nielsen; Kjeld Møllgård; Christian A Clement; Iben R Veland; Aashir Awan; Bradley K Yoder; Ivana Novak; Søren Tvorup Christensen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  237     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-04     Completed Date:  2008-10-23     Revised Date:  2012-11-19    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2039-52     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2008 Wiley-Liss, Inc.
Department of Biology, University of Copenhagen, Copenhagen, Denmark.
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MeSH Terms
Adenocarcinoma / metabolism,  pathology
Carcinoma, Pancreatic Ductal / metabolism*,  pathology
Cell Line, Tumor
Cilia / metabolism*
Epithelial Cells / metabolism,  ultrastructure
Fetus / cytology
Green Fluorescent Proteins / genetics
Hedgehog Proteins / metabolism*
Kruppel-Like Transcription Factors / genetics,  metabolism
NIH 3T3 Cells
Nuclear Proteins / genetics,  metabolism
Pancreas / cytology*,  embryology*,  metabolism
Pancreatic Neoplasms / metabolism*,  pathology
Receptors, Cell Surface / metabolism
Grant Support
Reg. No./Substance:
0/GLI2 protein, human; 0/Hedgehog Proteins; 0/Kruppel-Like Transcription Factors; 0/Nuclear Proteins; 0/Receptors, Cell Surface; 0/SHH protein, human; 0/SMO protein, human; 0/patched receptors; 147336-22-9/Green Fluorescent Proteins

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