Document Detail


Characterization of poly(ethylene glycol)-modified bovine hemoglobin by capillary zone electrophoresis.
MedLine Citation:
PMID:  17364466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study capillary zone electrophoresis (CZE) was used to analyze the poly(ethylene glycol)-modified bovine hemoglobin(PEG-bHb). The results show that CZE separated the subunit of bovine hemoglobin based on the number of PEG conjugating to the protein surface, which makes it possible to evaluate the degree of modification of hemoglobin subunit; meanwhile, it also reflected the stability of PEG attaching to hemoglobin after incubating with hydroxylamine, which makes it successful to detect the distribution of attachment site and evaluate the stability of PEG on the surface of hemoglobin. As a simple, fast and accurate method, CZE is suitable to monitor the production procedures and quality control of the final products of the PEG-bHb.
Authors:
Xian-Zhong Li; Qin-Hong Yang; Jiu-Feng Yan; Ke Xu; Jia Liu; Xue-Ying He; Xiao-Wei Zhang; Qian Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Artificial cells, blood substitutes, and immobilization biotechnology     Volume:  35     ISSN:  1073-1199     ISO Abbreviation:  Artif Cells Blood Substit Immobil Biotechnol     Publication Date:  2007  
Date Detail:
Created Date:  2007-03-16     Completed Date:  2007-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431307     Medline TA:  Artif Cells Blood Substit Immobil Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Affiliation:
Beijing Kaizheng Biotech Developing Ltd., Beijing, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Substitutes / chemical synthesis,  chemistry*
Cattle
Drug Stability
Electrophoresis, Capillary / methods*
Hemoglobins / chemistry*
Polyethylene Glycols / chemistry*
Protein Subunits / chemistry
Quality Control
Chemical
Reg. No./Substance:
0/Blood Substitutes; 0/Hemoglobins; 0/Polyethylene Glycols; 0/Protein Subunits

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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