| Characterization of polar lipids of oral isolates of Candida, Pichia and Saccharomyces by Fast Atom Bombardment Mass Spectrometry (FAB MS). | |
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MedLine Citation:
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PMID: 11309082 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To characterize fatty acid and phospholipid analogue profiles of oral yeasts. METHODS AND RESULTS: Twenty-seven strains of oral yeasts were cultured on SDA and lipids of freeze-dried cells were extracted and analysed by FAB MS. The most abundant carboxylate anion was m/z 281 (C18 : 1). The most intense phospholipid analogue ions were of PE, PG, PA and PI. Pichia etchellsii contained molecular species of PG and PE, whereas Saccharomyces cerevisiae had PA, PG and PE analogues. Mass spectra revealed that S. cerevisiae and Candida glabrata were distinct from one another and from the other species tested. CONCLUSION: Oral yeasts largely differ with respect to their polar lipids. It is concluded that oral yeast species have distinctive fatty acid and phospholipid analogue anion profiles. SIGNIFICANCE AND IMPACT OF THE STUDY: FAB MS provided novel chemotaxonomic information. |
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Authors:
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A Z Mahmoudabadi; V Boote; D B Drucker |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied microbiology Volume: 90 ISSN: 1364-5072 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-04-19 Completed Date: 2001-06-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 668-75 Citation Subset: IM |
Affiliation:
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Oral Microbiology Laboratory, School of Biological Sciences, University of Manchester, Manchester M15 6FH, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anions
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chemistry Candida / chemistry* Carboxylic Acids / chemistry Fatty Acids / analysis* Humans Mouth / microbiology Phospholipids / analysis* Pichia / chemistry* Saccharomyces / chemistry* Spectrometry, Mass, Fast Atom Bombardment / methods |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Carboxylic Acids; 0/Fatty Acids; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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