Document Detail


Characterization of the plasma membrane localization and orientation of HPV16 E5 for cell-cell fusion.
MedLine Citation:
PMID:  19712955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human papillomavirus (HPV) is a non-enveloped DNA virus with an approximately 8000 base pair genome. Infection with certain types of HPV is associated with cervical cancer, although the molecular mechanism by which HPV induces carcinogenesis is poorly understood. Three genes encoded by HPV16 are regarded as oncogenic - E5, E6, and E7. The role of E5 has been controversial. Expression of HPV16 E5 causes cell-cell fusion, an event that can lead to increased chromosomal instability, particularly in the presence of cell cycle checkpoint inhibitors like HPV16 E6 and E7. Using biochemical and cell biological assays to better understand HPV16 E5, we find that HPV16 E5 localizes to the plasma membrane with an intracellular amino terminus and an extracellular carboxyl-terminus. Further, HPV16 E5 must be expressed on both cells for cell fusion to occur. When the extracellular epitope of HPV16 E5 is targeted with an antibody, the number of bi-nucleated cells decreases.
Authors:
Lulin Hu; Brian P Ceresa
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-08-26
Journal Detail:
Title:  Virology     Volume:  393     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2009-10-09     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  135-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Fusion*
Cell Membrane / chemistry*
Human papillomavirus 16 / physiology*
Humans
Oncogene Proteins, Viral / analysis*,  physiology*
Grant Support
ID/Acronym/Agency:
P20 RR 017703/RR/NCRR NIH HHS; P20 RR017703/RR/NCRR NIH HHS; P20 RR017703-07/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Oncogene Proteins, Viral; 0/oncogene protein E5, Human papillomavirus type 16
Comments/Corrections

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