Document Detail

Characterization of plant proliferating cell nuclear antigen (PCNA) and flap endonuclease-1 (FEN-1), and their distribution in mitotic and meiotic cell cycles.
MedLine Citation:
PMID:  11851910     Owner:  NLM     Status:  MEDLINE    
The biochemical and cell cycle-dependent properties of proliferating cell nuclear antigen (OsPCNA) and flap endonuclease-1 (OsFEN-1) were characterized from rice (Oryza sativa). OsPCNA was physically associated with OsFEN-1 and increased the flap-endonuclease activity of OsFEN-1 by 2.5-fold. Northern and Western blotting analysis revealed that OsPCNA and OsFEN-1 were present in meristematic tissues such as cultured cells, shoot apical meristem and root apical meristem. No expression was detected in the mature leaves, although they were exposed to UV. Both of these proteins were localized in the nuclei of the interphase cells including G1, S and G2, and in the nuclear region at telophase. The distribution patterns of plant PCNA and FEN-1 in meiotic cell progression were investigated using microsporocytes of lily (Lilium longiflorum cv. Hinomoto). During the leptotene to pachytene stages, PCNA and FEN-1 were localized in the nuclear region. The florescence gradually disappeared from diplotene to metaphase I. Interestingly, signals for PCNA formed 10-20 intense spots at leptotene. The number of spots decreased to 1-5 at zygotene and finally to 1 at pachytene. The roles of OsPCNA and OsFEN-1 in mitotic and meiotic cell cycles are discussed.
S Kimura; T Suzuki; Y Yanagawa; T Yamamoto; H Nakagawa; I Tanaka; J Hashimoto; K Sakaguchi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  28     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2002-02-19     Completed Date:  2002-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  643-53     Citation Subset:  IM    
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278, Japan.
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MeSH Terms
Antibodies / immunology
Cell Line
Cross Reactions
Endodeoxyribonucleases / metabolism*
Flap Endonucleases
Plants / cytology,  enzymology*,  immunology*
Proliferating Cell Nuclear Antigen / metabolism*
Subcellular Fractions / enzymology,  immunology
Reg. No./Substance:
0/Antibodies; 0/Proliferating Cell Nuclear Antigen; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.-/Flap Endonucleases

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