| Characterization of phospholipase A2 activity in rat aorta smooth muscle cells. | |
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MedLine Citation:
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PMID: 6824708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phospholipase A activity was measured in homogenates and acid extracts of smooth muscle cells from rat aorta and mesenteric artery using [1-14 C]oleate-labeled autoclaved Escherichia coli and 1-[1-14C]stearyl-2-acyl-3-sn-glycerophosphorylethanolamine as substrates. The results demonstrate the presence of neutral-active phospholipase(s) A that exclusively catalyze the release of fatty acid from the 2-position of phospholipids. Optimal activity was at pH 7.5, and there was an absolute requirement for low concentrations of Ca2+. Mg2+ did not substitute for Ca2+, and EGTA inhibited the activity. Phospholipase A2 activity was predominantly membrane-associated and was solubilized by homogenization in 0.18 N H2SO4. Sulfuric acid extracts of rat aortic smooth muscle cells were four times more active than extracts of mesenteric artery (710 vs. 170 nmol/h per mg protein). By comparison, acid extracts of rat lung, heart, and liver were less active (60-75 nmol/h per mg). Indomethacin, sodium meclofenamate, mepacrine and chlorpromazine, but not dexamethasone or aspirin, inhibited acid-solubilized phospholipase(s) A2 between 10(-6) and 10(-3) M in a dose-dependent manner. Preincubation with p-bromophenacyl bromide or diethylpyrocarbonate inhibited phospholipase(s) A2, suggesting the presence of a histidine residue at the active site. An extract from the leaves of feverfew plant (Tanacetum parthenium) was also a potent inhibitor of aortic smooth muscle phospholipase(s) A2. |
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Authors:
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J K Thakkar; N Sperelakis; D Pang; R C Franson |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 750 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1983 Jan |
Date Detail:
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Created Date: 1983-04-15 Completed Date: 1983-04-15 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 134-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / enzymology Hydrogen-Ion Concentration Kinetics Liver / enzymology Lung / enzymology Mesenteric Arteries / enzymology Muscle, Smooth, Vascular / enzymology* Myocardium / enzymology Organ Specificity Phospholipases / metabolism* Phospholipases A / metabolism* Phospholipases A2 Plants, Medicinal Rats Sesquiterpenes Tanacetum parthenium |
| Grant Support | |
ID/Acronym/Agency:
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HL-07244/HL/NHLBI NIH HHS; HL-19242/HL/NHLBI NIH HHS; HL-24907/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Sesquiterpenes; EC 3.1.-/Phospholipases; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
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