| Characterization of peritoneal dialysis effluent-derived cells: diagnosis of peritoneal integrity. | |
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MedLine Citation:
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PMID: 23274627 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To assess the integrity of the peritoneal membrane, we characterized the functionality of the cellular components derived from peritoneal dialysis effluent (PDE). About 3 % of all cells collected from the PDE attached to the plastic dish, and 97.1 ± 3.1 % of the adherent cells expressed CK-18 (PDE-HPMC). A typical cobble-stone-like morphology under neutralized PD solution was obtained over 65 out of 74 primary cultures (88 %) while only 53 % under acidic PD solution in a previous report by Yanez-Mo et al. However, 26.6 ± 10.3 % of PDE-HPMC expressed the EMT marker α-SMA. Transepithelial resistance (TER) as a marker of cell polarity was 34 % lower than that of omentum-derived(OM)-HPMC. We found a significant decrease in the rate of PDE-HPMC proliferation compared to OM-HPMC, accompanied by a significant increase of cell area within the tertiary passage. Comparison of TER, α-SMA and SA-β-Gal between CAPD durations suggests that cell polarity weakens with increased duration of CAPD, reflecting the occurrence of EMT and cell senescence. We conclude that functional characterization of cellular components in PDE reflects how well the peritoneum is preserved. |
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Authors:
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Yo Higashi; Koji Abe; Tomoaki Kuzumoto; Takuya Hara; Keiichi Miyamoto; Tomohiro Murata; Eiji Ishikawa; Shinsuke Nomura; Takashi Horiuchi |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-30 |
Journal Detail:
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Title: Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs Volume: - ISSN: 1619-0904 ISO Abbreviation: J Artif Organs Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815648 Medline TA: J Artif Organs Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Division of Chemistry for Materials, Faculty of Engineering, Graduate School of Mie University, 1577 Kurima-Machiyacho, Tsu, Mie, 514-8507, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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