| Characterization of a pectic polysaccharide from the leaves of Diospyros kaki and its modulating activity on lymphocyte proliferation. | |
| | |
MedLine Citation:
|
PMID: 20235231 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Pectin is a group of carbohydrate polymers constructing the primary cell walls and the middle lamella of terrestrial plants. Herein, we demonstrated the structure and immunomodulatory activity of the major pectic polysaccharide DL-3B(2) isolated from the leaves of Diospyros kaki. Based on composition analysis, methylation analysis, two-step acid hydrolysis, lithium-mediated selective degradation, (13)C NMR spectroscopy, and electrospray ionization mass spectrometry, DL-3B(2) was found to contain an alpha-1, 4-linked galacturonic acid (GalA) backbone with some insertions of alpha-1, 2-linked rhamnose residues. The arabinan- and arabinogalactan-side chains were attached to O-4 of the rhamnose residues, whereas the linear arabinoxylan was probably linked to O-3 of the GalA residues. Immunological tests in vitro showed that DL-3B(2) could help stimulate lipopolysaccharide-induced B lymphocyte proliferation, but not ConA-induced T lymphocyte proliferation, and that the arabinose residues play a role in maintaining this immunological activity. |
| | |
Authors:
|
Jinyou Duan; Qun Dong; Kan Ding; Jinian Fang |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biopolymers Volume: 93 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-07 Completed Date: 2010-07-13 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
|
Languages: eng Pagination: 649-56 Citation Subset: IM |
Affiliation:
|
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China. jduan@rics.bwh.harvard.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals B-Lymphocytes / cytology, metabolism* Carbohydrate Conformation Carbohydrate Sequence Cell Proliferation / drug effects* Cells, Cultured Diospyros / chemistry* Food Additives / analysis, chemistry, isolation & purification, pharmacology Mice Pectins* / analysis, chemistry, isolation & purification, pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Food Additives; 0/Pectins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Atomic resolution crystallography of a complex of triosephosphate isomerase with a reaction-intermed...
Next Document: Expression analysis of TALE family transcription factors during avian development.