| Characterization of oxidative stress in Leishmaniasis-infected or LPS-stimulated macrophages using electrochemical impedance spectroscopy. | |
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MedLine Citation:
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PMID: 20488689 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The physiological changes caused by external stimuli can be employed as parameters to study pathogen infection in cells and the effect of drugs. Among analytical methods, impedance is potentially useful to give insight into cellular behavior by studying morphological changes, alterations in the physiological state, production of charged or redox species without interfering with in vitro cellular metabolism and labeling. The present work describes the use of electrochemical impedance spectroscopy to simply monitor by modeling impedance plots (Nyquist diagram) in appropriate equivalent circuit, the changes affecting murine macrophage cell line (RAW 264.7) in response to parasite infection by Leishmania amazonensis or to lipopolysaccharide (LPS) treatment. These results demonstrate the ability of electrochemical impedance spectroscopy to discriminate between two opposite cell responses associated to two different stimuli, one caused by the internalization of a parasite, and the other by activation by a bacterium component. Indeed, the study has allowed the characterization, from an electrical point of view, of the extra-cellular NO radical produced endogenously and in great quantities by the inducible form of NO-synthase in the case of LPS-stimulated macrophages. This production was not observed in the case of Leishmania-infected macrophages for which to survive and multiply, the parasite itself possesses mechanisms which may interfere with NO production. In this latest case, only the intracellular production of ROS was observed. To confirm these interpretations confocal microscopy analysis using the ROS (reactive oxygen species) fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate and electron paramagnetic resonance experiments using Fe(DETC)(2) as NO radical spin trap were carried out. |
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Authors:
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Karine Reybier; Clotilde Ribaut; Agnès Coste; Jérôme Launay; Paul Louis Fabre; Françoise Nepveu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-21 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 25 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 2566-72 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Université de Toulouse, UPS, LPSNPR (Laboratoire pharmacochimie des substances naturelles et pharmacophores redox), Toulouse, France. reybier@cict.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Electric Impedance Electrochemical Techniques Electron Spin Resonance Spectroscopy Host-Parasite Interactions Leishmania mexicana / pathogenicity Leishmaniasis / metabolism*, parasitology Lipopolysaccharides / pharmacology Macrophage Activation Macrophages / drug effects, metabolism*, parasitology* Mice Microscopy, Confocal Models, Biological Oxidative Stress* / drug effects Spectrum Analysis / methods |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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