Document Detail


Characterization of osmotically induced filaments of Salmonella enterica.
MedLine Citation:
PMID:  22798362     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella enterica forms aseptate filaments with multiple nucleoids when cultured in hyperosmotic conditions. These osmotic-induced filaments are viable and form single colonies on agar plates even though they contain multiple genomes and have the potential to divide into multiple daughter cells. Introducing filaments that are formed during osmotic stress into culture conditions without additional humectants results in the formation of septa and their division into individual cells, which could present challenges to retrospective analyses of infectious dose and risk assessments. We sought to characterize the underlying mechanisms of osmotic-induced filament formation. The concentration of proteins and chromosomal DNA in filaments and control cells was similar when standardized by biomass. Furthermore, penicillin-binding proteins in the membrane of salmonellae were active in vitro. The activity of penicillin-binding protein 2 was greater in filaments than in control cells, suggesting that it may have a role in osmotic-induced filament formation. Filaments contained more ATP than did control cells in standardized cell suspensions, though the levels of two F(0)F(1)-ATP synthase subunits were reduced. Furthermore, filaments could septate and divide within 8 h in 0.2 × Luria-Bertani broth at 23°C, while nonfilamentous control cells did not replicate. Based upon the ability of filaments to septate and divide in this diluted broth, a method was developed to enumerate by plate count the number of individual, viable cells within a population of filaments. This method could aid in retrospective analyses of infectious dose of filamented salmonellae.
Authors:
Zachary L Pratt; Bingming Chen; Charles J Czuprynski; Amy C L Wong; Charles W Kaspar
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-13
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-27     Completed Date:  2012-12-12     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6704-13     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analysis
Bacterial Proteins / analysis
Culture Media / chemistry
DNA, Bacterial / analysis
Osmotic Pressure*
Penicillin-Binding Proteins / analysis
Proton-Translocating ATPases / analysis
Salmonella enterica / chemistry,  cytology*,  growth & development,  physiology*
Stress, Physiological*
Temperature
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/Penicillin-Binding Proteins; 56-65-5/Adenosine Triphosphate; EC 3.6.3.14/Proton-Translocating ATPases
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