| Characterization of oscillations of intracellular calcium concentration in ferret ventricular muscle. | |
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MedLine Citation:
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PMID: 6747885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The photoprotein aequorin was injected into superficial cells of ferret papillary muscles. Tension and aequorin light (a function of intracellular [Ca2+]) were monitored. Increasing intracellular Ca concentration ([Ca2+]i), either by decreasing extracellular Na, or by inhibiting the Na pump with strophanthidin, produced spontaneous oscillations of [Ca2+]i and tension. Fourier analysis showed that these oscillations had frequencies of up to 3-4 Hz. If the muscle was stimulated in these conditions the Ca transient associated with the twitch was followed by a series of damped oscillations of [Ca2+]i which were accompanied by after-contractions. Under a given set of conditions the frequency of the stimulated oscillations was similar to that of the spontaneous oscillations. Manoeuvres which increase [Ca2+]i increased the frequency of both spontaneous and stimulated oscillations. Drugs which inhibit the function of the sarcoplasmic reticulum (caffeine and ryanodine) abolished both stimulated and spontaneous oscillations. The spontaneous oscillations during a Na-free contracture were unaffected by the Ca channel blocker D-600. When repetitive stimulation was begun the frequency and magnitude of the stimulated oscillations increased over several minutes. Increasing the frequency of stimulation increased the magnitude of the stimulated oscillations. It is concluded that the spontaneous oscillations of [Ca2+]i may be due to oscillatory Ca release from the sarcoplasmic reticulum. The similar properties of the spontaneous and stimulated oscillations suggest that the latter may be due to a synchronization of the former. |
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Authors:
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D G Allen; D A Eisner; C H Orchard |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of physiology Volume: 352 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-09-19 Completed Date: 1984-09-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 113-28 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aequorin
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diagnostic use Animals Caffeine / pharmacology Calcium / metabolism* Carnivora / metabolism* Ferrets / metabolism* Muscle Contraction / drug effects Muscles / drug effects, metabolism* Papillary Muscles / metabolism Potassium / pharmacology Sarcoplasmic Reticulum / drug effects, metabolism Sodium / pharmacology Strophanthidin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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HL 12186/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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50934-79-7/Aequorin; 58-08-2/Caffeine; 66-28-4/Strophanthidin; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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