Document Detail


Characterization of one newly established esophageal cancer cell line CSEC from a high-incidence area in China.
MedLine Citation:
PMID:  18477252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Chaoshan littoral is located in a high-incidence area of esophageal cancer in the south of China. In this study, a new esophageal cancer cell line CSEC was established from a 47-year-old female Chinese patient in this district. The biological characters of the cultured cells were investigated, including morphology, ultrastructure, growth kinetic features, tumorigenicity, expression of tumor-associated antigen and cytogenetic features. CSEC cell line grew continuously with a doubling time of 39.5 h and had been passaged over 80 times. The CSEC cells possessed features of squamous epithelial cells with cytokeratin indicated by immunohistochemical staining and tonofilaments and desmosomes revealed by electron microscopy. Tumorigenicity to severe combined immunodeficient mice was confirmed and the tumors developed revealed well-differentiated squamous cell carcinoma, similar to the origin tumor from which the cell line derived. The cytogenetic analysis demonstrated hypertetraploid karyotypes. Chromosome structure aberrations were common and complicated. Immunohistochemical staining showed that CSEC cells were infected with HPV and over-expressed p53. In summary, the CSEC cell line is a well-differentiated esophageal squamous cell carcinoma cell line from a high-incidence area in southern China. It may provide a useful model for the pathogenesis and therapeutic research of esophageal squamous cell carcinoma.
Authors:
S-M Lu; M Su; D-P Tian; W-D Deng; Y-L Zheng; H-H Huang; M-H Chen; X-Y Li
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus / I.S.D.E     Volume:  21     ISSN:  1442-2050     ISO Abbreviation:  Dis. Esophagus     Publication Date:  2008  
Date Detail:
Created Date:  2008-05-14     Completed Date:  2008-06-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809160     Medline TA:  Dis Esophagus     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  309-15     Citation Subset:  IM    
Affiliation:
Department of Pathology, Shantou University Medical College, Guangdong Province, China.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / epidemiology,  physiopathology*
Cell Line, Tumor
China / epidemiology
Esophageal Neoplasms / epidemiology,  physiopathology*
Humans
Incidence

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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