| Characterization of oncogene dysregulation in multiple myeloma by combined FISH and DNA microarray analyses. | |
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MedLine Citation:
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PMID: 15543617 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chromosomal translocations involving the immunoglobulin heavy chain (IGH) locus and various partner loci frequently are associated with multiple myeloma (MM). We investigated the expression profiles of the FGFR3/MMSET, CCND1, CCND3, MAF, and MAFB genes, which are involved in t(4;14)(p16.3;q32), t(11;14)(q13;q32), t(6;14)(p21;q32), t(14;16)(q32;q23), and t(14;20)(q32;q12), respectively, in purified plasma cell populations from 39 MMs and six plasma cell leukemias (PCL) by DNA microarray analysis and compared the results with the presence of translocations as assessed by dual-color FISH or RT-PCR. A t(4;14) was found in 6 MMs, t(11;14) in 9 MMs and 1 PCL, t(6;14) in 1 MM, t(14;16) in 2 MMs and 1 PCL, and t(14;20) in 1 PCL. In all cases, the translocations were associated with the spiked expression of target genes. Furthermore, gene expression profiling enabled the identification of putative translocations causing dysregulation of CCND1 (1 MM and 1 PCL) and MAFB (1 MM and 1 PCL) without any apparent involvement of immunoglobulin loci. Notably, all of the translocations were mutually exclusive. Markedly increased MMSET expression was found in 1 MM showing associated FGFR3 and MMSET signals on an unidentified chromosome. Our data suggest the importance of using combined molecular cytogenetic and gene expression approaches to detect genetic aberrations in MM. |
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Authors:
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Sonia Fabris; Luca Agnelli; Michela Mattioli; Luca Baldini; Domenica Ronchetti; Fortunato Morabito; Donata Verdelli; Lucia Nobili; Daniela Intini; Vincenzo Callea; Caterina Stelitano; Luigia Lombardi; Antonino Neri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genes, chromosomes & cancer Volume: 42 ISSN: 1045-2257 ISO Abbreviation: Genes Chromosomes Cancer Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2004-12-13 Completed Date: 2005-07-19 Revised Date: 2012-06-05 |
Medline Journal Info:
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Nlm Unique ID: 9007329 Medline TA: Genes Chromosomes Cancer Country: United States |
Other Details:
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Languages: eng Pagination: 117-27 Citation Subset: IM |
Copyright Information:
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(c) 2004 Wiley-Liss, Inc. |
Affiliation:
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Laboratorio di Ematologia Sperimentale e Genetica Molecolare, UO Ematologia 1, Dipartimento di Scienze Mediche, Università degli Studi di Milano, Ospedale Maggiore IRCCS, Milan, Italy. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Carrier Proteins / genetics Chromosomes, Human, Pair 11 / genetics Chromosomes, Human, Pair 14 / genetics Chromosomes, Human, Pair 16 / genetics Chromosomes, Human, Pair 20 / genetics Chromosomes, Human, Pair 4 / genetics Chromosomes, Human, Pair 6 / genetics Cyclin D1 / genetics Cyclin D3 Cyclins / genetics DNA-Binding Proteins / genetics Female Gene Expression Profiling / methods* Gene Expression Regulation, Neoplastic / physiology* Histone-Lysine N-Methyltransferase Humans In Situ Hybridization, Fluorescence / methods* Macrophage-Activating Factors / genetics MafB Transcription Factor Male Microarray Analysis / methods* Middle Aged Multiple Myeloma / genetics* Oncogene Proteins / genetics Oncogene Proteins, Fusion Oncogenes / genetics* Protein-Tyrosine Kinases / genetics Receptor, Fibroblast Growth Factor, Type 3 Receptors, Fibroblast Growth Factor / genetics Repressor Proteins / genetics Transcription Factors / genetics Translocation, Genetic / genetics |
| Chemical | |
Reg. No./Substance:
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0/CCND3 protein, human; 0/Carrier Proteins; 0/Cyclin D3; 0/Cyclins; 0/DNA-Binding Proteins; 0/MAFB protein, human; 0/Macrophage-Activating Factors; 0/MafB Transcription Factor; 0/Oncogene Proteins; 0/Oncogene Proteins, Fusion; 0/Receptors, Fibroblast Growth Factor; 0/Repressor Proteins; 0/Transcription Factors; 136601-57-5/Cyclin D1; EC 2.1.1.43/Histone-Lysine N-Methyltransferase; EC 2.1.1.43/WHSC1 protein, human; EC 2.7.10.1/FGFR3 protein, human; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Receptor, Fibroblast Growth Factor, Type 3 |
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