Document Detail

Characterization of oligosaccharide moieties of intact glycoproteins by microwave-assisted partial acid hydrolysis and mass spectrometry.
MedLine Citation:
PMID:  16124037     Owner:  NLM     Status:  MEDLINE    
A method, which utilizes microwave-assisted partial acid hydrolysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), to elucidate oligosaccharide composition of intact glycoproteins is presented here. Glycoproteins, such as ribonuclease B, avidin, alpha1-acid glycoprotein, and fetuin, are used as model systems to demonstrate this technique. Partial cleavage of oligosaccharides from whole intact glycoproteins with trifluoroacetic acid was observed after a short exposure to microwaves. Due to the high-resolution mass spectra obtained by MALDI-TOFMS from glycoproteins with molecular weights less than 20 kDa, the compositions of oligosaccharides are readily derived for ribonuclease B and avidin. The data agree with the proposed oligosaccharide structures of ribonuclease B (five glycoforms) and avidin (eight glycoforms). Larger glycoproteins such as alpha1-acid glycoprotein (many glycoforms) and fetuin (many glycoforms) exhibited only broad peaks with no glycoform resolution. Nevertheless, this method can be used successfully for analysis of glycoproteins with molecular weights greater than 20 kDa to determine the presence or absence of glycosylation.
Bao-Shiang Lee; Sangeeth Krishnanchettiar; Syed Salman Lateef; Nabila Salman Lateef; Shalini Gupta
Related Documents :
16351927 - Sialic acids: fascinating sugars in higher animals and man.
29007 - Adherence of mycoplasma gallisepticum to human erythrocytes.
7581337 - Lipophosphoglycan is present in distinctly different form in different entamoeba histol...
19576577 - A lectin from the chinese bird-hunting spider binds sialic acids.
15181417 - Ethyl pyruvate: a novel treatment for sepsis and shock.
21319737 - Method to attenuate u(vi) mobility in acidic waste plumes using humic acids.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  19     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2005  
Date Detail:
Created Date:  2005-09-05     Completed Date:  2006-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  2629-35     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 John Wiley & Sons, Ltd.
Protein Research Laboratory, Research Resources Center, 835 S. Wolcott Avenue, University of Illinois, Chicago, IL 60612, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acids / chemistry
Amino Acid Sequence
Avidin / chemistry
Egg White
Glycoproteins / chemistry*
Hydrogen-Ion Concentration
Molecular Sequence Data
Oligosaccharides / analysis*,  chemistry*
Ribonucleases / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Reg. No./Substance:
0/Acids; 0/Glycoproteins; 0/Oligosaccharides; 1405-69-2/Avidin; EC 3.1.-/Ribonucleases; EC 3.1.27.-/ribonuclease B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Analyzing glycerol-mediated protein oligomerization by electrospray ionization mass spectrometry.
Next Document:  Characterization of carotenoids and carotenoid esters in red pepper pods (Capsicum annuum L.) by hig...