Document Detail


Characterization of the oligosaccharide component of arylsulfatase B from rat liver.
MedLine Citation:
PMID:  9360706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A glycan chain analysis of the total oligosaccharide pool derived from rat liver arylsulfatase B was carried out by. P4 Gel Permeation Chromatography and sequential exoglycosidase digestion. It was found that 71% of rat liver arylsulfatase B oligosaccharides were sialylated. The relative contribution of particular structures in the total glycan pool was as follows: sialylated biantennary complex type glycans with terminal galactose--65%, high-mannose type glycans--15%, biantennary complex type glycans with core fucose and terminal N-acetylglucosamine--5%, O-linked oligosaccharides--3.5%.
Authors:
M Przybyło; A Lityńska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  44     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  1997  
Date Detail:
Created Date:  1998-02-19     Completed Date:  1998-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  181-90     Citation Subset:  IM    
Affiliation:
Department of Animal Physiology, Jagiellonian University, Cracow, Poland. kloc@zuk.iz.uj.edu.pl
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MeSH Terms
Descriptor/Qualifier:
Animals
Chondro-4-Sulfatase / analysis*,  isolation & purification
Chromatography, Paper / methods
Electrophoresis, Polyacrylamide Gel
Liver / enzymology*
Male
Oligosaccharides / analysis*,  isolation & purification
Polysaccharides / analysis,  isolation & purification
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Oligosaccharides; 0/Polysaccharides; EC 3.1.6.9/Chondro-4-Sulfatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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