| Characterization of oil bodies in jelly fig achenes. | |
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MedLine Citation:
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PMID: 18434174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thin-layer chromatography analysis revealed that the contents stored in oil bodies isolated from jelly fig (Ficus awkeotsang Makino) achenes were mainly neutral lipids (>90% triacylglycerols and approximately 5% diacylglycerols). Fatty acids released from the neutral lipids of achene oil bodies were highly unsaturated (62.65% alpha-linolenic acid, 18.24% linoleic acid, and 10.62% oleic acid). The integrity of isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms and one caleosin were present in these oil bodies. MALDI-MS analyses confirmed that the three full-length cDNA fragments obtained by PCR cloning from maturing achenes encoded the two jelly fig oleosin isoforms and one caleosin identified by immunological screening. |
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Authors:
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Anna C N Chua; Pei-Luen Jiang; Li-Shian Shi; Wing-Ming Chou; Jason T C Tzen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-18 |
Journal Detail:
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Title: Plant physiology and biochemistry : PPB / Société française de physiologie végétale Volume: 46 ISSN: 0981-9428 ISO Abbreviation: Plant Physiol. Biochem. Publication Date: 2008 May-Jun |
Date Detail:
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Created Date: 2008-05-30 Completed Date: 2008-09-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882449 Medline TA: Plant Physiol Biochem Country: France |
Other Details:
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Languages: eng Pagination: 525-32 Citation Subset: IM |
Affiliation:
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Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40227, Taiwan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EF583922; EF583923; EU157185 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Blotting, Western Chromatography, Thin Layer Diglycerides / chemistry, metabolism Electrophoresis, Polyacrylamide Gel Fatty Acids / chemistry, metabolism Ficus / genetics, metabolism* Inclusion Bodies / chemistry, metabolism* Linoleic Acid / chemistry, metabolism Molecular Sequence Data Oleic Acid / chemistry, metabolism Plant Oils / chemistry, metabolism* Plant Proteins / chemistry, genetics, metabolism Polymerase Chain Reaction Protein Isoforms / chemistry, genetics, metabolism Seeds / genetics, metabolism* Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Triglycerides / chemistry, metabolism alpha-Linolenic Acid / chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Diglycerides; 0/Fatty Acids; 0/Plant Oils; 0/Plant Proteins; 0/Protein Isoforms; 0/Triglycerides; 112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; 463-40-1/alpha-Linolenic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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