Document Detail


Characterization of the oat1 gene of Penicillium chrysogenum encoding an omega-aminotransferase: induction by L-lysine, L-ornithine and L-arginine and repression by ammonium.
MedLine Citation:
PMID:  16163487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Penicillium chrysogenum oat1 gene, which encodes a class III omega-aminotransferase, was cloned and characterized. This enzyme converts lysine into 2-aminoadipic semialdehyde, and plays an important role in the biosynthesis of 2-aminoadipic acid, a precursor of penicillin and other beta-lactam antibiotics. The enzyme is related to ornithine-5-aminotransferases and to the lysine-6-aminotransferases encoded by the lat genes found in bacterial cephamycin gene clusters. Expression of oat1 is induced by lysine, ornithine and arginine, and repressed by ammonium ions. AreA-binding GATA and GATT sequences involved in regulation by ammonium, and an 8-bp direct repeat associated with arginine induction in Emericella (Aspergillus nidulans and Saccharomyces cerevisiae, were found in the oat1 promoter region. Deletion of the oat1 gene resulted in the loss of omega-aminotransferase activity. The null mutants were unable to grow on ornithine or arginine as sole nitrogen sources and showed reduced growth on lysine. Complementation of the null mutant with the oat1 gene restored normal levels of omega-aminotransferase activity and the ability to grow on ornithine, arginine and lysine. The role of the oat1 gene in the biosynthesis of 2-aminoadipic acid is discussed.
Authors:
Leopoldo Naranjo; Mònica Lamas-Maceiras; Ricardo V Ullán; Sonia Campoy; Fernando Teijeira; Javier Casqueiro; Juan F Martín
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-20
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  274     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-20     Completed Date:  2006-05-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  283-94     Citation Subset:  IM    
Affiliation:
Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, Spain.
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MeSH Terms
Descriptor/Qualifier:
2-Aminoadipic Acid / biosynthesis*
Amino Acid Sequence
Arginine / pharmacology
Base Sequence
Blotting, Northern
Cloning, Molecular
Conserved Sequence / genetics
DNA Primers
DNA, Complementary / genetics
Fungal Proteins / genetics
Gene Deletion
Gene Expression Regulation, Bacterial / drug effects*,  genetics
Introns / genetics
Lysine / metabolism*,  pharmacology
Molecular Sequence Data
Mutation / genetics
Ornithine / pharmacology
Penicillium chrysogenum / enzymology*,  growth & development
Promoter Regions, Genetic / genetics
Quaternary Ammonium Compounds / toxicity
Sequence Analysis, DNA
Transaminases / genetics*,  metabolism*
Transcription Factors / genetics
Chemical
Reg. No./Substance:
0/AreA protein, Aspergillus nidulans; 0/DNA Primers; 0/DNA, Complementary; 0/Fungal Proteins; 0/Quaternary Ammonium Compounds; 0/Transcription Factors; 542-32-5/2-Aminoadipic Acid; 56-87-1/Lysine; 7006-33-9/Ornithine; 74-79-3/Arginine; EC 2.6.1.-/Transaminases

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