| Characterization of a novel rat epididymal cell line to study epididymal function. | |
| | |
MedLine Citation:
|
PMID: 16099865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The epididymis is an androgen-dependent organ that allows spermatozoa to become fully functional as they pass through this tissue. The specialized functions of the epididymis are mediated by interactions between epididymal epithelial cells and between epididymal cells and spermatozoa. Although the critical role of the epididymis in sperm maturation is well established, the mechanisms regulating cell-cell interactions remain poorly understood because of the lack of appropriate cell line models. We now report the characterization of a novel rat caput epididymal cell line (RCE) that was immortalized by transfecting primary cultures of rat epididymal cells with the simian virus 40 large T antigen. At the electron microscope level, the cell line was composed of epithelial principal cells with characteristics of in vivo cells; principal cells had well-developed Golgi apparatus, abundant endoplasmic reticulum cisternae, and few endosomes. RCE cells expressed the mRNAs coding for the androgen receptor, estrogen receptor alpha, and 4-ene-steroid-5-alpha-reductase types 1 and 2 as well as epididymal-specific markers Crisp-1 and epididymal retinoic acid binding protein. Epididymal retinoic acid binding protein expression was significantly induced with dihydrotestosterone, although this effect was not blocked by flutamide, suggesting that RCE cells are not androgen responsive. Neighboring cells formed tight and gap junctions characteristic of epididymal cells in vivo and expressed tight (occludin and claudin-1, -3, and -4) and gap junctional proteins (connexin-26, -30.3, -32, and -43). The RCE cell line displays many characteristics of epithelial principal cells, thus providing a model for studying epididymal cell functions. |
| | |
Authors:
|
Julie Dufresne; Nancy St-Pierre; Robert S Viger; Louis Hermo; Daniel G Cyr |
Related Documents
:
|
11082325 - Connexin-independent ganciclovir-mediated killing conferred on bystander effect-resista... 18506685 - Lysophosphatidic acid induces ovarian cancer cell dispersal by activating fyn kinase as... 12198655 - Expression and regulation of gap junctions in rat cholangiocytes. 11683185 - Regulation of cell morphology and cytochrome p450 expression in human hepatocytes by ex... 7507215 - Measurement and characterization of micronuclei in exfoliated human cells by fluorescen... 20354915 - Hyperpolarization induces differentiation in human cardiomyocyte progenitor cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-08-11 |
Journal Detail:
|
Title: Endocrinology Volume: 146 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2005 Nov |
Date Detail:
|
Created Date: 2005-10-17 Completed Date: 2005-12-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
|
Languages: eng Pagination: 4710-20 Citation Subset: AIM; IM |
Affiliation:
|
Institut National de la Recherche Scientifique-Institut Armand Frappier, Université du Québec, 245 Hymus Boulevard, Pointe Claire, Quebec, Canada H9R 1G6. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Androgens
/
pharmacology Animals Antigens, Viral, Tumor / genetics Biological Markers / metabolism Cell Line, Transformed* Epididymis / cytology*, drug effects, physiology*, ultrastructure Gap Junctions / metabolism Male Microscopy, Electron Proteins / metabolism Rats Rats, Sprague-Dawley Simian virus 40 / immunology Tight Junctions / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
|
0/Androgens; 0/Antigens, Viral, Tumor; 0/Biological Markers; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Estrogens up-regulate the Fas/FasL apoptotic pathway in lactotropes.
Next Document: Ciprofibrate stimulates the gastrin-producing cell by acting luminally on antral PPAR-alpha.