| Characterization of a novel polymeric scaffold for potential application in tendon/ligament tissue engineering. | |
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MedLine Citation:
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PMID: 16499446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Unlike braided fabrics, knitted scaffolds have been proven to favor deposition of collagenous connective tissue matrix, which is crucial for tendon/ligament reconstruction. But cell seeding of such scaffolds often requires a gel system, which is unstable in a dynamic situation, especially in the knee joint. This study developed a novel, biodegradable nano-microfibrous polymer scaffold by electrospinning PLGA nanofibers onto a knitted PLGA scaffold in order to provide a large biomimetic surface for cell attachment. Porcine bone marrow stromal cells were seeded onto either the novel scaffolds by pipetting a cell suspension (Group I) or the knitted PLGA scaffolds by immobilizing in fibrin gel (Group II). Cell attachment at 36 hours, cell proliferation and extracellular matrix synthesis at 1 week, and mechanical properties over 2 weeks were investigated. Cell attachment was comparable and cell proliferation was faster in Group I. Moreover, cellular function was more actively exhibited in Group I, as evident by the higher expression of collagen I, decorin, and biglycan genes. Thus, this novel scaffold, facilitating cell seeding and promoting cell proliferation, function, and differentiation, could be applied with promise in tissue engineering of tendon/ligament. |
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Authors:
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S Sahoo; H Ouyang; James C-H Goh; T E Tay; S L Toh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tissue engineering Volume: 12 ISSN: 1076-3279 ISO Abbreviation: Tissue Eng. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-02-27 Completed Date: 2006-05-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9505538 Medline TA: Tissue Eng Country: United States |
Other Details:
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Languages: eng Pagination: 91-9 Citation Subset: IM |
Affiliation:
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Tissue Repair Lab, Division of Bioengineering, National University of Singapore, Singapore. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biocompatible Materials* Bone Marrow Cells Cells, Cultured Extracellular Matrix Proteins / genetics Lactic Acid* Ligaments* / ultrastructure Materials Testing Microscopy, Confocal Nanostructures Polyglycolic Acid* Polymers* Reverse Transcriptase Polymerase Chain Reaction Stromal Cells Swine Tendons* / ultrastructure Tissue Engineering* |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Extracellular Matrix Proteins; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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