Document Detail


Characterization of novel O-glycans isolated from tear and saliva of ocular rosacea patients.
MedLine Citation:
PMID:  23294139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
O-Glycans in saliva and tear isolated from patients suffering from ocular rosacea, a form of inflammatory ocular surface disease, were profiled, and their structures were elucidated using high resolution mass spectrometry. We have previously shown that certain structures, particularly sulfated oligosaccharides, increased in the tear and saliva of rosacea patients. In this study, the structures of these glycans were elucidated using primarily tandem mass spectrometry. There were important similarities in the glycan profiles of tears and saliva with the majority of the structures in common. The structures of the most abundant species common to both tear and saliva, which were also the most abundant species in both, were elucidated. For sulfated species, the positions of the sulfate groups were localized. The majority of the structures were new, with the sulfated glycans comprising mucin core 1- and core 2-type structures. As both saliva and tear are rich in mucins, it is suggested that the O-glycans are mainly components of mucins. The study further illustrates the strong correspondence between the glycans in the tear and saliva of ocular rosacea patients.
Authors:
Sureyya Ozcan; Hyun Joo An; Ana C Vieira; Gun Wook Park; Jae Han Kim; Mark J Mannis; Carlito B Lebrilla
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-02-06
Journal Detail:
Title:  Journal of proteome research     Volume:  12     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-01     Completed Date:  2013-08-29     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1090-100     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid
Humans
Polysaccharides / isolation & purification*
Rosacea / metabolism*
Saliva / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tears / metabolism*
Grant Support
ID/Acronym/Agency:
R01 GM049077/GM/NIGMS NIH HHS; R01GM049077/GM/NIGMS NIH HHS; S10 RR027639/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Polysaccharides
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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