Document Detail


Characterization of a novel microsomal fatty acid synthetase (FAS) compared to a cytosolic FAS in the housefly, Musca domestica.
MedLine Citation:
PMID:  9440236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel membrane-bound fatty acid synthetase (FAS) associated with the microsomal fraction from the housefly, Musca domestica, was solubilized and purified to homogeneity. The microsomal FAS was solubilized by 0.75 M KCl in phosphate buffer and was purified to homogeneity by the sequential use of ammonium sulfate precipitation followed by Sepharose CL-6B, DEAE Sephacel and Red Agarose (dye ligand affinity) chromatography. The specific activity of the microsomal FAS was increased 1,440-fold to 6,522 U/mg during purification. The cytosolic FAS from the housefly was also purified by similar methods and the specific activity increased 183-fold to 7,533 U/mg. The relative molecular mass of the microsomal and cytosolic FAS are 419 +/- 22 kDa and 405 +/- 18 kDa, respectively, for the dimers as determined by gel permeation chromatography. The microsomal and the cytosolic FAS yield different tryptic digestion maps and have slightly different amino acid compositions, which demonstrate structural differences between the two FASs. In addition, there are differences between the two FASs in their kinetic characteristics and their ability to incorporate methylmalonylCoA into the growing fatty acyl chain.
Authors:
P Gu; W H Welch; L Guo; K M Schegg; G J Blomquist
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  118     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1998-02-24     Completed Date:  1998-02-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  447-56     Citation Subset:  IM    
Affiliation:
Department of Biochemistry/MS 330, University of Nevada, Reno 89557-0014, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / metabolism
Amino Acids / analysis*
Ammonium Sulfate / pharmacology
Animals
Chemical Precipitation
Cytosol / enzymology*
Fatty Acid Synthetase Complex / chemistry*,  drug effects,  metabolism*
Female
Houseflies / enzymology*
Kinetics
Microsomes / enzymology*
Molecular Weight
Potassium Chloride / pharmacology
Solubility
Trypsin / chemistry
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Amino Acids; 1264-45-5/methylmalonyl-coenzyme A; 7447-40-7/Potassium Chloride; 7783-20-2/Ammonium Sulfate; EC 3.4.21.4/Trypsin; EC 6.-/Fatty Acid Synthetase Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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