| Characterization of a novel lipoxygenase-independent senescence mechanism in Alstroemeria peruviana floral tissue. | |
| | |
MedLine Citation:
|
PMID: 12226507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The role of lipoxygenase (lox) in senescence of Alstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products generated. Phospholipids and galactolipids were extensively degraded during senescence in both sepals and petals and the ratio of saturated/unsaturated fatty acids increased. Lox protein levels and enzymatic activity declined markedly after flower opening. Stereochemical analysis of lox products showed that 13-lox was the major activity present in both floral tissues and high levels of 13-keto fatty acids were also synthesized. Lipid hydroperoxides accumulated in sepals, but not in petals, and sepals also had a higher chlorophyll to carotenoid ratio that favors photooxidation of lipids. Loss of membrane semipermeability was coincident for both tissue types and was chronologically separated from lox activity that had declined by over 80% at the onset of electrolyte leakage. Thus, loss of membrane function was not related to lox activity or accumulation of lipid hydroperoxides per se and differs in these respects from other ethylene-insensitive floral tissues representing a novel pattern of flower senescence. |
| | |
Authors:
|
Michael K Leverentz; Carol Wagstaff; Hilary J Rogers; Anthony D Stead; Usawadee Chanasut; Helena Silkowski; Brian Thomas; Heiko Weichert; Ivo Feussner; Gareth Griffiths |
Related Documents
:
|
18631507 - The resistance of epicuticular components of an insect to extraction with lipid solvents. 9751667 - Mycobacterial lipids: a historical perspective. 803497 - Phosphatidylkojibiosyl diglyceride. the covalently linked lipid constituent of the memb... 12562857 - Quantification of phosphatidic acid and lysophosphatidic acid by hplc with evaporative ... 2776127 - Structure of the capsular polysaccharide of klebsiella ozaenae serotype k4 containing 3... 16717417 - Accumulation of gamma-aminobutyric acid in rice germ using protease. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Plant physiology Volume: 130 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 2002 Sep |
Date Detail:
|
Created Date: 2002-09-12 Completed Date: 2002-12-09 Revised Date: 2010-09-14 |
Medline Journal Info:
|
Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 273-83 Citation Subset: IM |
Affiliation:
|
Department of Plant Genetics and Biotechnology, Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, United Kingdom. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiosperms
/
enzymology*,
growth & development Anthocyanins / metabolism Antioxidants / metabolism Carotenoids / metabolism Chlorophyll / metabolism Electrolytes / metabolism Fatty Acids / chemistry, metabolism Lipid Metabolism Lipid Peroxidation Lipids / chemistry Lipoxygenase / metabolism* Molecular Conformation Pigments, Biological / metabolism Plant Stems / enzymology* Reactive Oxygen Species / metabolism Thiobarbituric Acid Reactive Substances / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Anthocyanins; 0/Antioxidants; 0/Electrolytes; 0/Fatty Acids; 0/Lipids; 0/Pigments, Biological; 0/Reactive Oxygen Species; 0/Thiobarbituric Acid Reactive Substances; 1406-65-1/Chlorophyll; 36-88-4/Carotenoids; EC 1.13.11.12/Lipoxygenase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Plasmalemma abscisic acid perception leads to RAB18 expression via phospholipase D activation in Ara...
Next Document: Mitochondrial-driven bicarbonate transport supports photosynthesis in a marine microalga.