Document Detail


Characterization of a novel lipoxygenase-independent senescence mechanism in Alstroemeria peruviana floral tissue.
MedLine Citation:
PMID:  12226507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of lipoxygenase (lox) in senescence of Alstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products generated. Phospholipids and galactolipids were extensively degraded during senescence in both sepals and petals and the ratio of saturated/unsaturated fatty acids increased. Lox protein levels and enzymatic activity declined markedly after flower opening. Stereochemical analysis of lox products showed that 13-lox was the major activity present in both floral tissues and high levels of 13-keto fatty acids were also synthesized. Lipid hydroperoxides accumulated in sepals, but not in petals, and sepals also had a higher chlorophyll to carotenoid ratio that favors photooxidation of lipids. Loss of membrane semipermeability was coincident for both tissue types and was chronologically separated from lox activity that had declined by over 80% at the onset of electrolyte leakage. Thus, loss of membrane function was not related to lox activity or accumulation of lipid hydroperoxides per se and differs in these respects from other ethylene-insensitive floral tissues representing a novel pattern of flower senescence.
Authors:
Michael K Leverentz; Carol Wagstaff; Hilary J Rogers; Anthony D Stead; Usawadee Chanasut; Helena Silkowski; Brian Thomas; Heiko Weichert; Ivo Feussner; Gareth Griffiths
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant physiology     Volume:  130     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-12     Completed Date:  2002-12-09     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-83     Citation Subset:  IM    
Affiliation:
Department of Plant Genetics and Biotechnology, Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Angiosperms / enzymology*,  growth & development
Anthocyanins / metabolism
Antioxidants / metabolism
Carotenoids / metabolism
Chlorophyll / metabolism
Electrolytes / metabolism
Fatty Acids / chemistry,  metabolism
Lipid Metabolism
Lipid Peroxidation
Lipids / chemistry
Lipoxygenase / metabolism*
Molecular Conformation
Pigments, Biological / metabolism
Plant Stems / enzymology*
Reactive Oxygen Species / metabolism
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Anthocyanins; 0/Antioxidants; 0/Electrolytes; 0/Fatty Acids; 0/Lipids; 0/Pigments, Biological; 0/Reactive Oxygen Species; 0/Thiobarbituric Acid Reactive Substances; 1406-65-1/Chlorophyll; 36-88-4/Carotenoids; EC 1.13.11.12/Lipoxygenase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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