| Characterization of a novel autosomal dominant bleeding disorder in a large kindred from east Texas. | |
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MedLine Citation:
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PMID: 11238089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A large east Texas family with autosomal dominant inheritance of a novel bleeding disorder has been identified. The disorder is characterized clinically by easy bruising, life-threatening bleeding with trauma or surgery, and menorrhagia in affected women. Laboratory studies demonstrated prolongation of the prothrombin time and activated partial thromboplastin time in affected individuals. Paradoxically, assays of known coagulation factors are all within normal limits. To determine the molecular basis of this disease, a candidate gene linkage analysis in this kindred was done. Initially it was hypothesized that the cause of the disease in this family could be an antithrombin III (AT3) mutation that resulted in a constitutively active AT3 in the absence of heparin binding. Linkage studies using DNA from the family and an intragenic polymorphic marker within the AT3 gene showed that the disease mapped to this locus. The coding region and intron/exon junctions of AT3 were sequenced using the proband's DNA, but this analysis failed to identify a mutation. Additional family members were recruited for the study, and 16 polymorphic markers around the AT3 gene were analyzed. Using 2 recombinants, the critical interval for the defective gene was narrowed to approximately 1.5 Mb, centromeric to AT3. The factor V (FV) gene was mapped into the disease interval and sequenced; there were no mutations found. Elucidation of the genetic defect causing the bleeding disorder in this family may reveal a novel protein involved in the coagulation cascade. |
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Authors:
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S Q Kuang; S Hasham; M D Phillips; D Wolf; Y Wan; P Thiagarajan; D M Milewicz |
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Publication Detail:
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Type: Case Reports; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Blood Volume: 97 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-03-12 Completed Date: 2001-04-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 1549-54 Citation Subset: AIM; IM |
Affiliation:
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Department of Internal Medicine, University of Texas-Houston Medical School, Houston, TX 77030, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antithrombin III / genetics Blood Coagulation Factors Blood Coagulation Tests Chromosome Mapping / methods Chromosomes, Human, Pair 1 / genetics Factor V / genetics Family Health Female Genes, Dominant / genetics* Genetic Markers Haplotypes Hemorrhage / epidemiology, etiology, genetics* Humans Linkage (Genetics) Male Mutation Pedigree Texas / epidemiology |
| Grant Support | |
ID/Acronym/Agency:
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M01-RR02558/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Coagulation Factors; 0/Genetic Markers; 9000-94-6/Antithrombin III; 9001-24-5/Factor V |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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