Document Detail


Characterization of a novel EAST-negative enteropathogenic E. coli strain implicated in a food-borne outbreak of diarrhoea in adults.
MedLine Citation:
PMID:  23134539     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Enteropathogenic Escherichia coli (EPEC) is usually associated with outbreaks and sporadic cases of severe infantile diarrhoea in the developing world, and less commonly with sporadic cases in developed countries. Very little evidence indicates that EPEC is a food-borne pathogen for adults. In a previous study, two groups of adult travellers became ill, and eae(+) E. coli of serogroup O111 was isolated from affected individuals and epidemiologically linked to food consumption. Here the strain responsible was further investigated and characterized as an unusual atypical EPEC. PCR analysis of the designated type isolate showed the presence of the rorf1 and espB genes of the LEE pathogenicity island, which was inserted at the chromosomal selC locus. The isolate was negative for the enteroaggregative E. coli EAST-1 toxin present in other strains of EPEC associated with food-borne outbreaks. The strain adhered sparsely to HEp-2 cell monolayers in a diffuse manner, but fluorescent actin staining demonstrated that it was capable of inducing polymerization of actin at the sites of bacterial attachment. Strain P2583 is the first EAST-negative EPEC to be confirmed as a cause of outbreaks of infection in adults following the consumption of contaminated food or water.
Authors:
Amy L Wedley; Hasan M Elajnef; Jonathan N Fletcher
Related Documents :
10970369 - Molecular variability of pseudallescheria boydii, a neurotropic opportunist.
20937219 - Occurrence of pectobacterium carotovorum strains isolated from potato soft rot in morocco.
18993109 - Five-fold increase in trypanosoma congolense isolates resistant to diminazene aceturate...
9925589 - Genetic and morphological characterization of cladobotryum species causing cobweb disea...
18608919 - Genetic diversity among clinical coccidioides spp. isolates in arizona.
15900969 - Polymorphism of aeromonas spp. trna intergenic spacers.
18611799 - Microbial multidrug resistance.
2845759 - Trimethoprim/sulphamethoxazole resistance in escherichia coli and klebsiella spp. urina...
6290219 - Phosphoribosylpyrophosphate synthetase of escherichia coli, identification of a mutant ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  APMIS : acta pathologica, microbiologica, et immunologica Scandinavica     Volume:  -     ISSN:  1600-0463     ISO Abbreviation:  APMIS     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803400     Medline TA:  APMIS     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors APMIS © 2012 APMIS.
Affiliation:
School of Medical Sciences, University of Bradford, Bradford, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Tandem Catalysis for the Preparation of Cylindrical Polypeptide Brushes.
Next Document:  Elective segmental ureterectomy for transitional cell carcinoma of the ureter: long-term follow-up i...