Document Detail

Characterization of novel Brucella strains originating from wild native rodent species in North Queensland, Australia.
MedLine Citation:
PMID:  20639360     Owner:  NLM     Status:  MEDLINE    
We report on the characterization of a group of seven novel Brucella strains isolated in 1964 from three native rodent species in North Queensland, Australia, during a survey of wild animals. The strains were initially reported to be Brucella suis biovar 3 on the basis of microbiological test results. Our results indicated that the rodent strains had microbiological traits distinct from those of B. suis biovar 3 and all other Brucella spp. To reinvestigate these rodent strains, we sequenced the 16S rRNA, recA, and rpoB genes and nine housekeeping genes and also performed multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA). The rodent strains have a unique 16S rRNA gene sequence compared to the sequences of the classical Brucella spp. Sequence analysis of the recA, rpoB, and nine housekeeping genes reveals that the rodent strains are genetically identical to each other at these loci and divergent from any of the currently described Brucella sequence types. However, all seven of the rodent strains do exhibit distinctive allelic MLVA profiles, although none demonstrated an amplicon for VNTR 07, whereas the other Brucella spp. did. Phylogenetic analysis of the MLVA data reveals that the rodent strains form a distinct clade separate from the classical Brucella spp. Furthermore, whole-genome sequence comparison using the maximal unique exact matches index (MUMi) demonstrated a high degree of relatedness of one of the seven rodent Brucella strains (strain NF 2653) to another Australian rodent Brucella strain (strain 83-13). Our findings strongly suggest that this group of Brucella strains isolated from wild Australian rodents defines a new species in the Brucella genus.
Rebekah V Tiller; Jay E Gee; Michael A Frace; Trevor K Taylor; Joao C Setubal; Alex R Hoffmaster; Barun K De
Publication Detail:
Type:  Journal Article     Date:  2010-07-16
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-11-30     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5837-45     Citation Subset:  IM    
Centers for Disease Control and Prevention, Mail Stop G34, 1600 Clifton Road, Atlanta, GA 30333, USA.
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MeSH Terms
Bacterial Proteins / genetics
Bacterial Typing Techniques
Brucella / classification*,  isolation & purification*
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
DNA-Directed RNA Polymerases / genetics
RNA, Ribosomal, 16S / genetics
Rec A Recombinases / genetics
Rodentia / microbiology*
Sequence Analysis, DNA
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S; EC 2.7.7.-/Rec A Recombinases; EC RNA Polymerases; EC polymerase beta subunit

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