Document Detail

Characterization of a novel 14 kDa bile acid-binding protein from rat ileal cytosol.
MedLine Citation:
PMID:  1859823     Owner:  NLM     Status:  MEDLINE    
A 14 kDa polypeptide in rat ileal cytosol has been identified as the major intestinal cytosolic bile acid-binding protein (I-BABP) by photoaffinity labeling with the radiolabeled 7,7-azo derivative of taurocholate (7,7-azo-TC). To further characterize I-BABP, the protein was purified by lysylglycocholate Sepharose 4B affinity and DE-52 anion-exchange chromatography. The purified I-BABP contained a single 14 kDa band on SDS-PAGE. The 14 kDa protein showed a 26-fold increase in binding affinity for [3H]7,7-azo-TC compared to cytosolic protein. Immunoblotting of protein fractions separated by affinity chromatography showed that neither liver fatty acid binding protein (L-FABP) nor intestinal fatty acid binding protein (I-FABP) bind to the affinity column and that the 14 kDa protein which bound to the column and was subsequently eluted with detergent did not cross-react with anti-L-FABP or anti-I-FABP. The 14 kDa protein labeled with [3H]7,7-azo-TC was radioimmunoprecipitated from cytosol by rabbit antiserum raised against purified I-BABP. I-BABP was shown to have a blocked N-terminus; however, its mixed internal sequence generated from cyanogen bromide-cleaved protein and amino acid composition indicated that it was related to (although clearly distinct from) both I-FABP and L-FABP. These studies have isolated a 14 kDa bile acid-binding protein from rat ileal cytosol which is immunologically and biochemically distinct from I-FABP and L-FABP.
M C Lin; Y Z Gong; K F Geoghegan; F A Wilson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1078     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-09-04     Completed Date:  1991-09-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  329-35     Citation Subset:  IM    
Department of Medicine, Pennsylvania State University College of Medicine, Hershey.
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MeSH Terms
Amino Acid Sequence
Bile Acids and Salts / metabolism*
Carrier Proteins / isolation & purification*
Chromatography, Ion Exchange
Cytosol / chemistry*
Hydroxysteroid Dehydrogenases*
Ileum / chemistry*
Membrane Glycoproteins*
Molecular Sequence Data
Molecular Weight
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Bile Acids and Salts; 0/Carrier Proteins; 0/Membrane Glycoproteins; 0/bile acid binding proteins; EC 1.1.-/Hydroxysteroid Dehydrogenases; EC 1.1.1.-/AKR1C2 protein, human

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