Document Detail


Characterization of normal canine anterior cruciate ligament-associated synoviocytes.
MedLine Citation:
PMID:  18203201     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was designed to identify and quantify synoviocyte phenotypes enveloping the canine anterior cruciate ligament (ACL) to test the hypothesis that there are at least two synoviocyte phenotypes, each with distinct quantities and topographical distributions. CD18 and HSP25 epitopes were colocalized in the synovium of 10 normal canine ACLs. Sagittal sections were prepared from medial, central, and lateral aspects of each ACL and phenotypes were quantified in the proximal, middle, and distal aspects of each section. Distinct synoviocyte populations stained positive for CD18 (CD18+) or HSP25 (HSP25+), and a small population of cells stained for both epitopes (DS+). The proportion (mean +/- SEM) of HSP25+ synoviocytes (57% +/- 7.5%) was significantly greater than the proportion of CD18+ synoviocytes (27% +/- 8.2%), which was significantly greater than the proportion of DS+ synoviocytes (16% +/- 3.5%). Reverse transcriptase polymerase chain reaction (RT-PCR), Western blot analysis, and immunoelectron microscopy confirmed the presence of CD18 and HSP25 epitopes in the canine ACL. Identification and quantification of ACL synoviocytes may serve as the foundation for future studies involving ACL disease or reconstruction.
Authors:
Sunil C Vasanjee; Daniel Paulsen; Giselle Hosgood; Sandra O Robinson; Mandi J Lopez
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  26     ISSN:  1554-527X     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-20     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  809-15     Citation Subset:  IM    
Copyright Information:
(c) 2008 Orthopaedic Research Society.
Affiliation:
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Skip Bertman Drive, Louisiana State University, Baton Rouge, Louisiana 70803, USA. vasanjee@vetmed.lsu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Anterior Cruciate Ligament / cytology*,  metabolism
Antigens, CD18 / genetics,  metabolism
Dogs
Female
Fibroblasts / cytology*,  metabolism,  ultrastructure
Heat-Shock Proteins / genetics,  metabolism
Immunohistochemistry
Macrophages / cytology*,  metabolism,  ultrastructure
Male
Microscopy, Electron, Transmission
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Synovial Membrane / cytology*,  metabolism
Grant Support
ID/Acronym/Agency:
K01 AR002174-05/AR/NIAMS NIH HHS; K01 AR002174-06/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD18; 0/Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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