Document Detail

Characterization of the non-invasive assessment of the cutaneous microcirculation by laser Doppler perfusion scanner.
MedLine Citation:
PMID:  20618693     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Microcirculatory dysfunction contributes to morbidity and mortality in vascular diseases. Here, we aimed at establishing a sensitive and valid method to measure microvascular reactivity during post-occlusive reactive hyperemia (PORH) using scanning laser Doppler perfusion imaging (LDPI) of the forearm. METHODS: In a first series, LDPI was methodologically evaluated on the volar forearm of healthy volunteers (n = 10) before and after one to five minutes of upper arm occlusion. In a second series, readings were performed in 20 healthy subjects and 20 patients with coronary artery disease (CAD). RESULTS: Three minutes of forearm occlusion were sufficient to induce maximal vasodilation during PORH as indicated by maximal increase in perfusion unit (PU) amplitude that did not further increase after five-minute occlusion. Five-minute occlusion led to a significant prolongation of PORH with greater area under curve (AUC) suggesting longer lasting vasodilation of microvessels. The five-minute occlusion was associated with lower variability as compared with three minutes (intraindividual variability: 9-17% vs. 12-21%; interindividual variability: 13-24% vs. 14-26%). CAD patients exhibited significantly reduced amplitude (105 +/- 49 vs. 164 +/- 35 PU; p < 0.001), ratio (4.7 +/- 1.8 vs. 7.1 +/- 1.8; p < 0.001), and AUC (1656 +/- 1070 vs. 2723 +/- 864 PU x minutes; p = 0.001). CONCLUSION: Scanning LDPI is a feasible and reproducible method for non-invasive assessment of the cutaneous microcirculatory response during PORH.
Stefanie Keymel; Julia Sichwardt; Jan Balzer; Emilia Stegemann; Tienush Rassaf; Petra Kleinbongard; Malte Kelm; Christian Heiss; Thomas Lauer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  17     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  358-66     Citation Subset:  IM    
Department of Medicine, Division of Cardiology, Pneumology, and Vascular Diseases, University Hospital, Heinrich-Heine University Duesseldorf, Duesseldorf, Germany.
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MeSH Terms
Case-Control Studies
Coronary Artery Disease / physiopathology
Hyperemia / physiopathology
Laser-Doppler Flowmetry / methods*
Microcirculation / physiology*
Middle Aged
Reproducibility of Results
Skin / blood supply*
Vasodilation / physiology
Young Adult

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