Document Detail


Characterization of non-O157 shiga toxin-producing Escherichia coli isolates from healthy fat-tailed sheep in southeastern of Iran.
MedLine Citation:
PMID:  23015355     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objectives of this study were to determine the presence and prevalence of non-O157 shiga toxin-producing Escherichia coli (STEC) isolates from faeces of healthy fat-tailed sheep and detection of phylogenetic background and antibiotic resistance profile of isolates. One hundred ninety-two E. coli isolates were recovered from obtained rectal swabs and were confirmed by biochemical tests. Antibiotic resistance profiles of isolates were detected and phylogenetic background of isolates was determined according to the presence of the chuA, yjaA and TspE4.C2 genetic markers. The isolates were examined to determine stx ( 1 ), stx ( 2 ) and eae genes. Non-O157 STEC isolates were identified by using O157 specific antiserum. Forty-three isolates (22.40 %) were positive for one of the stx ( 1 ), stx ( 2 ) and eae genes, whereas 10.42 % were positive for stx ( 1 ), 19.38 % for eae and 2.60 % for stx ( 2 ) gene. None of the positive isolates belonged to O157 serogroup. Twenty isolates possessed stx ( 1 ) were distributed in A (six isolates), B1 (13) and D (one) phylogroups, whereas stx ( 2 ) positive isolates fell into A (three isolates) and B1 (two) phylogenetic groups. Eighteen isolates contained eae gene belonged to A (five isolates), B1 (seven) and D (six) phylogroups. The maximum and minimum resistance rates were recorded against to penicillin and co-trimoxazole respectively. The positive isolates for stx ( 1 ), stx ( 2 ) and eae genes showed several antibiotic resistance patterns, whereas belonged to A, B1 and D phylogroups. In conclusion, faeces of healthy sheep could be considered as the important sources of non-O157 STEC and also multidrug-resistant E. coli isolates.
Authors:
Reza Ghanbarpour; Mojtaba Kiani
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Tropical animal health and production     Volume:  -     ISSN:  1573-7438     ISO Abbreviation:  Trop Anim Health Prod     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1277355     Medline TA:  Trop Anim Health Prod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Microbiology Department of Faculty of Veterinary Medicine, Shahid Bahonar University, 76169-14111, Kerman, Iran, ghanbar@mail.uk.ac.ir.
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