Document Detail


Characterization of nifB, nifS, and nifU genes in the cyanobacterium Anabaena variabilis: NifB is required for the vanadium-dependent nitrogenase.
MedLine Citation:
PMID:  7883714     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anabaena variabilis ATCC 29413 is a heterotrophic, nitrogen-fixing cyanobacterium containing both a Mo-dependent nitrogenase encoded by the nif genes and V-dependent nitrogenase encoded by the vnf genes. The nifB, nifS, and nifU genes of A. variabilis were cloned, mapped, and partially sequenced. The fdxN gene was between nifB and nifS. Growth and acetylene reduction assays using wild-type and mutant strains indicated that the nifB product (NifB) was required for nitrogen fixation not only by the enzyme encoded by the nif genes but also by the enzyme encoded by the vnf genes. Neither NifS nor NifU was essential for nitrogen fixation in A. variabilis.
Authors:
E M Lyons; T Thiel
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  177     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-04-13     Completed Date:  1995-04-13     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1570-5     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Missouri--St. Louis 63121.
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MeSH Terms
Descriptor/Qualifier:
Acetylene / metabolism
Anabaena / genetics*,  metabolism
Bacterial Proteins / genetics*
Cloning, Molecular
Genes, Bacterial*
Genetic Complementation Test
Mutagenesis
Nitrogen Fixation / genetics
Nitrogenase / genetics*,  metabolism
Restriction Mapping
Transcription, Genetic
Vanadium / metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/NifB protein, Bacteria; 0/NifU protein, Bacteria; 0/nifS protein, Bacteria; 74-86-2/Acetylene; 7440-62-2/Vanadium; EC 1.18.6.1/Nitrogenase
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