Document Detail


Characterization of a new small cell lung cancer (SCLC) cell line STP54 derived from a metastatic bioptate of a combined type of SCLC with Non-SCLC component.
MedLine Citation:
PMID:  19419948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Small cell lung cancer constitutes 15-20% cases of lung cancers, currently the leading cause of death from malignant diseases. It also causes the demise of >90% of affected individuals in 5 years. We have established a new SCLC cell line STP54 derived from fine needle aspirate of metastatic supraclavicular lymph node of 54 -year-old women for model experiments. The primary tumor was diagnosed by histopathological examination as combined type of small cell lung cancer with a non-small cell component. We cultured the cancer cells in the RPMI 1640 medium. In the long-term culture only the small cell component survived. The cell line was established after 30 passages and then characterized by performing cell morphology, cell growth analysis, tumorigenicity in vitro and flow cytometry analysis of selected markers (like NCAM, cytokeratines, HLA-ABC, Fas, Bcl-2, p53, CXCR4, CD210). The cells were growing in floating aggregates and show features suggesting its invasiveness. We suggest that this new cell line may serve as a valuable tool for further studies on lung tumor biology, molecular pathogenesis and metastatic mechanism.
Authors:
Tomasz Skirecki; Grazyna Hoser; Joanna Domagała-Kulawik; Jerzy Kawiak
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  47     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-05-07     Completed Date:  2009-07-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  111-5     Citation Subset:  IM    
Affiliation:
Department of Clinical Cytology, Medical Center of Postgraduate Education, Warsaw, Poland.
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MeSH Terms
Descriptor/Qualifier:
Biopsy
Carcinoma, Non-Small-Cell Lung / pathology*
Cell Aggregation
Cell Line, Tumor
Cell Proliferation
Female
Flow Cytometry
Humans
Immunohistochemistry
Immunophenotyping
Kinetics
Lung Neoplasms / pathology*
Lymphatic Metastasis / pathology*
Middle Aged
Small Cell Lung Carcinoma / pathology*
Tumor Markers, Biological / metabolism
Tumor Stem Cell Assay
Chemical
Reg. No./Substance:
0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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