| Characterization of new isolated Ralstonia eutropha strain A-04 and kinetic study of biodegradable copolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate) production. | |
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MedLine Citation:
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PMID: 18712546 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new isolated bacterial strain A-04 capable of producing high content of polyhydroxyalkanoates (PHAs) was morphologically and taxonomically identified based on biochemical tests and 16S rRNA gene analysis. The isolate is a member of the genus Ralstonia and close to Ralstonia eutropha. Hence, this study has led to the finding of a new and unexplored R. eutropha strain A-04 capable of producing PHAs with reasonable yield. The kinetic study of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] production by the R. eutropha strain A-04 was examined using butyric acid and gamma-hydroxybutyric acid as carbon sources. Effects of substrate ratio and mole ratio of carbon to nitrogen (C/N) on kinetic parameters were investigated in shake flask fed-batch cultivation. When C/N was 200, that is, nitrogen deficient condition, the specific production rate of 3-hydroxybutyrate (3HB) showed the highest value, whereas when C/N was in the range between 4 and 20, the maximum specific production rate of 4-hydroxybutyrate (4HB) was obtained. Thus, the synthesis of 3HB was growth-limited production under nitrogen-deficient condition, whereas the synthesis of 4HB was growth-associated production under nitrogen-sufficient condition. The mole fraction of 4HB units increased proportionally as the ratio of gamma-hydroxybutyric acid in the feed medium increased at any value of C/N ratio. Based on these kinetic studies, a simple strategy to improve P(3HB-co-4HB) production in shake flask fed-batch cultivation was investigated using C/N and substrate feeding ratio as manipulating variable, and was successfully proved by the experiments. |
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Authors:
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Suchada Chanprateep; Yoshio Katakura; Sirirat Visetkoop; Hiroshi Shimizu; Songsri Kulpreecha; Suteaki Shioya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-20 |
Journal Detail:
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Title: Journal of industrial microbiology & biotechnology Volume: 35 ISSN: 1367-5435 ISO Abbreviation: J. Ind. Microbiol. Biotechnol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-14 Completed Date: 2009-03-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 1205-15 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai, Patumwan, Bangkok, 10330, Thailand. suchada.cha@chula.ac.th |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EF988626 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biodegradation, Environmental Cupriavidus necator / classification, genetics, isolation & purification*, metabolism* Hydroxybutyrates / chemistry*, metabolism* Kinetics Molecular Sequence Data Phylogeny Polyesters / chemistry*, metabolism* Soil Microbiology |
| Chemical | |
Reg. No./Substance:
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0/Hydroxybutyrates; 0/Polyesters; 117068-64-1/poly(3-hydroxybutyrate-co-4-hydroxybutyrate) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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