Document Detail

Characterization of new isolated Ralstonia eutropha strain A-04 and kinetic study of biodegradable copolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate) production.
MedLine Citation:
PMID:  18712546     Owner:  NLM     Status:  MEDLINE    
A new isolated bacterial strain A-04 capable of producing high content of polyhydroxyalkanoates (PHAs) was morphologically and taxonomically identified based on biochemical tests and 16S rRNA gene analysis. The isolate is a member of the genus Ralstonia and close to Ralstonia eutropha. Hence, this study has led to the finding of a new and unexplored R. eutropha strain A-04 capable of producing PHAs with reasonable yield. The kinetic study of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] production by the R. eutropha strain A-04 was examined using butyric acid and gamma-hydroxybutyric acid as carbon sources. Effects of substrate ratio and mole ratio of carbon to nitrogen (C/N) on kinetic parameters were investigated in shake flask fed-batch cultivation. When C/N was 200, that is, nitrogen deficient condition, the specific production rate of 3-hydroxybutyrate (3HB) showed the highest value, whereas when C/N was in the range between 4 and 20, the maximum specific production rate of 4-hydroxybutyrate (4HB) was obtained. Thus, the synthesis of 3HB was growth-limited production under nitrogen-deficient condition, whereas the synthesis of 4HB was growth-associated production under nitrogen-sufficient condition. The mole fraction of 4HB units increased proportionally as the ratio of gamma-hydroxybutyric acid in the feed medium increased at any value of C/N ratio. Based on these kinetic studies, a simple strategy to improve P(3HB-co-4HB) production in shake flask fed-batch cultivation was investigated using C/N and substrate feeding ratio as manipulating variable, and was successfully proved by the experiments.
Suchada Chanprateep; Yoshio Katakura; Sirirat Visetkoop; Hiroshi Shimizu; Songsri Kulpreecha; Suteaki Shioya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-20
Journal Detail:
Title:  Journal of industrial microbiology & biotechnology     Volume:  35     ISSN:  1367-5435     ISO Abbreviation:  J. Ind. Microbiol. Biotechnol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-14     Completed Date:  2009-03-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705544     Medline TA:  J Ind Microbiol Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1205-15     Citation Subset:  IM    
Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai, Patumwan, Bangkok, 10330, Thailand.
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MeSH Terms
Biodegradation, Environmental
Cupriavidus necator / classification,  genetics,  isolation & purification*,  metabolism*
Hydroxybutyrates / chemistry*,  metabolism*
Molecular Sequence Data
Polyesters / chemistry*,  metabolism*
Soil Microbiology
Reg. No./Substance:
0/Hydroxybutyrates; 0/Polyesters; 117068-64-1/poly(3-hydroxybutyrate-co-4-hydroxybutyrate)

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