Document Detail

Characterization of a new gene WX2 in Toxoplasma gondii.
MedLine Citation:
PMID:  17627323     Owner:  NLM     Status:  MEDLINE    
Using hybridization techniques, we prepared the monoclonal antibody (Mab) 7C3-C3 against Toxoplasma gondii. The protection tests showed that the protein (Mab7C3-C3) inhibited the invasion and proliferation of T. gondii RH strain in HeLa cells. The passive transfer test indicated that the antibody significantly prolonged the survival time of the challenged mice. It was also shown that the antibody could be used for the detection of the circulating antigen of T. gondii. After immunoscreening the T. gondii tachyzoite cDNA library with Mab7C3-C3, a new gene wx2 of T. gondii was obtained. Immunofluorescence analysis showed that the WX2 protein was located on the membrane of the parasite. Nucleotide sequence comparison showed 28% identity to the calcium channel alpha-1E unit and shared with the surface antigen related sequence in some conservative residues. However, no match was found in protein databases. Therefore, it was an unknown gene in T. gondii encoding a functional protein on the membrane of T. gondii. Because it has been shown to have a partial protective effect against T. gondii infection and is released as a circulating antigen, it could be a candidate molecule for vaccine or a novel target for new drugs.
Xiang Wu; Qiong Zhang; Kui Tan; Ronghua Xie; Jiubo Fan; Hengping Shu; Shiping Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biochimica et biophysica Sinica     Volume:  39     ISSN:  1672-9145     ISO Abbreviation:  Acta Biochim. Biophys. Sin. (Shanghai)     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-13     Completed Date:  2007-09-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101206716     Medline TA:  Acta Biochim Biophys Sin (Shanghai)     Country:  China    
Other Details:
Languages:  eng     Pagination:  475-83     Citation Subset:  IM    
Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Genes, Protozoan*
Mice, Inbred BALB C
Molecular Sequence Data
Toxoplasma / genetics*,  immunology,  pathogenicity
Toxoplasmosis / parasitology*

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