Document Detail

Characterization of the neurons of the mouse hypogastric ganglion: morphology and electrophysiology.
MedLine Citation:
PMID:  2341700     Owner:  NLM     Status:  MEDLINE    
The somata of mouse hypogastric ganglion cells injected with Lucifer yellow were ovoid in shape, lacked dendritic processes but gave rise to a single axonal process. Antidromic activation demonstrated that some of the cells contained in this ganglion innervated the vas deferens. The passive and active membrane properties of the ganglion cells were determined in current clamp experiments. Cells fired tetrodotoxin-sensitive action potentials in response to intracellularly-applied depolarizing current. In voltage clamp evidence was obtained for both a persistent inward calcium current at potentials between -30 and -40 mV and a transient calcium current evoked by step depolarizations to around -20 mV. In current clamp, however, cells did not fire calcium action potentials in the presence of tetrodotoxin. Three potassium currents, IM (blocked by 1 mM barium and by 30 microM bethanechol), IA (blocked by 2 mM 4-aminopyridine) and IK(Ca) fast (blocked by 100 microM cadmium and by 5 mM tetraethylammonium) were characterized in these neurons. In addition, IK(Ca) slow was observed in a small proportion of cells. Fast, all-or-nothing, excitatory synaptic potentials were recorded in response to single stimuli applied to the afferent fibres running to the ganglion. In most cells the excitatory synaptic potentials were suprathreshold for action potential initiation and were markedly reduced or abolished by 100 microM mecamylamine, 1 mM hexamethonium and following desensitization to 100 microM nicotine. Excitatory synaptic potentials arose from stimulation of a single presynaptic nerve process and are typical of strong synaptic inputs.
H Rogers; C Kennedy; G Henderson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the autonomic nervous system     Volume:  29     ISSN:  0165-1838     ISO Abbreviation:  J. Auton. Nerv. Syst.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-06-26     Completed Date:  1990-06-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003419     Medline TA:  J Auton Nerv Syst     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  255-70     Citation Subset:  IM    
Department of Pharmacology, University of Cambridge, U.K.
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MeSH Terms
Action Potentials / drug effects
Autonomic Nervous System / drug effects,  physiology*
Calcium / physiology
Electric Stimulation
Hypogastric Plexus / drug effects,  physiology*
Mecamylamine / pharmacology
Membrane Potentials / drug effects
Mice, Inbred DBA
Potassium / physiology
Tetraethylammonium Compounds / pharmacology
Vas Deferens / innervation*
Reg. No./Substance:
0/Tetraethylammonium Compounds; 60-40-2/Mecamylamine; 7440-09-7/Potassium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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