Document Detail


Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum.
MedLine Citation:
PMID:  20346355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the adult teleost brain, proliferating cells are observed in a broad area, while these cells have a restricted distribution in adult mammalian brains. In the adult teleost optic tectum, most of the proliferating cells are distributed in the caudal margin of the periventricular gray zone (PGZ). We found that the PGZ is largely divided into 3 regions: 1 mitotic region and 2 post-mitotic regions-the superficial and deep layers. These regions are distinguished by the differential expression of several marker genes: pcna, sox2, msi1, elavl3, gfap, fabp7a, and s100beta. Using transgenic zebrafish Tg (gfap:GFP), we found that the deep layer cells specifically express gfap:GFP and have a radial glial morphology. We noted that bromodeoxyuridine (BrdU)-positive cells in the mitotic region did not exhibit glial properties, but maintained neuroepithelial characteristics. Pulse chase experiments with BrdU-positive cells revealed the presence of self-renewing stem cells within the mitotic region. BrdU-positive cells differentiate into glutamatergic or GABAergic neurons and oligodendrocytes in the superficial layer and into radial glial cells in the deep layer. These results demonstrate that the proliferating cells in the PGZ contribute to neuronal and glial lineages to maintain the structure of the optic tectum in adult zebrafish.
Authors:
Yoko Ito; Hideomi Tanaka; Hitoshi Okamoto; Toshio Ohshima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-24
Journal Detail:
Title:  Developmental biology     Volume:  342     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-10     Completed Date:  2010-06-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26-38     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Life Science and Medical Bio-Science, School of Advanced Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, 162-8480, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Animals, Genetically Modified
Brain / cytology,  metabolism*
Bromodeoxyuridine / metabolism
Cell Differentiation / genetics,  physiology
Glial Fibrillary Acidic Protein / genetics,  metabolism
Green Fluorescent Proteins / genetics,  metabolism
Humans
Multipotent Stem Cells / metabolism
Neuroglia / cytology,  metabolism*
Neurons / cytology,  metabolism*
Stem Cells / cytology,  metabolism*,  physiology
Superior Colliculi / metabolism*
Zebrafish / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 147336-22-9/Green Fluorescent Proteins; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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