| Characterization of natural hybrids of Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum. | |
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MedLine Citation:
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PMID: 17302940 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nine yeast strains were isolated from spontaneous fermentations in the Alsace area of France, during the 1997, 1998 and 1999 grape harvests. Strains were characterized by pulsed-field gel electrophoresis, PCR-restriction fragment length polymorphism (RFLP) of the MET2 gene, delta-PCR, and microsatellite patterns. Karyotypes and MET2 fragments of the nine strains corresponded to mixed chromosomal bands and restriction patterns for both Saccharomyces cerevisiae and Saccharomyces bayanus var. uvarum. They also responded positively to amplification with microsatellite primers specific to both species and were demonstrated to be diploid. However, meiosis led to absolute nonviability of their spores on complete medium. All the results demonstrated that the nine yeast strains isolated were S. cerevisiaexS. bayanus var. uvarum diploid hybrids. Moreover, microsatellite DNA analysis identified strains isolated in the same cellar as potential parents belonging to S. bayanus var. uvarum and S. cerevisiae. |
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Authors:
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Christine Le Jeune; Marc Lollier; Catherine Demuyter; Claude Erny; Jean-Luc Legras; Michel Aigle; Isabelle Masneuf-Pomarède |
Publication Detail:
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Type: Journal Article Date: 2007-02-16 |
Journal Detail:
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Title: FEMS yeast research Volume: 7 ISSN: 1567-1356 ISO Abbreviation: FEMS Yeast Res. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-05-21 Completed Date: 2007-07-31 Revised Date: 2008-10-07 |
Medline Journal Info:
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Nlm Unique ID: 101085384 Medline TA: FEMS Yeast Res Country: England |
Other Details:
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Languages: eng Pagination: 540-9 Citation Subset: IM |
Affiliation:
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Laboratoire Vigne Biotechnologie et Environnement, Université de Haute-Alsace, Herrlisheim, Colmar, France. christine.le-jeune@uha.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Fungal
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genetics* Fermentation France Hybridization, Genetic* Karyotyping Microsatellite Repeats Mycological Typing Techniques Polymerase Chain Reaction Saccharomyces / classification*, genetics, isolation & purification, metabolism Saccharomyces cerevisiae / classification*, genetics, isolation & purification, metabolism Wine / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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