Document Detail


Characterization of myosin-A and myosin-C: two class XIV unconventional myosins from Toxoplasma gondii.
MedLine Citation:
PMID:  10470019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two class XIV unconventional myosins from Toxoplasma gondii, Myosin-A (TgM-A) and Myosin-C (TgM-C), were characterized in terms of their biochemical properties and their expression in quiescent and motile stages of the parasite life cycle. In cell fractionation studies, both myosins partitioned with the major organelle/cell membrane fraction, and extraction studies indicated that both were tightly associated with membrane domains as detergent was necessary for their solubilization. In addition, both TgM-A and TgM-C demonstrated a hallmark feature of myosins in their ability to bind actin in the absence but not the presence of ATP. In parasites residing within the host cell parasitophorous vacuole, TgM-A was detected by immunofluorescence microscopy as a bright spot near the apical pole of the parasite. This pattern underwent a subtle change as the parasites became motile, with TgM-A then localizing more intimately with the parasite cell membrane domain in apically disposed spots or patches, consistent with the role of this myosin in gliding motility. TgM-C showed a distinct localization to the juxtanuclear region towards the apical pole of the parasite, consistent with an association with the Golgi apparatus.
Authors:
M B Heintzelman; J D Schwartzman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  44     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  1999  
Date Detail:
Created Date:  1999-10-01     Completed Date:  1999-10-01     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  58-67     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Wiley-Liss, Inc.
Affiliation:
Department of Anatomy, Dartmouth Medical School, Hanover, New Hampshire, USA. heintzelman@dartmouth.edu
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Adenosine Triphosphate / pharmacology
Animals
Antibodies / immunology
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique, Indirect
Myosins / analysis*,  immunology,  metabolism
Protein Binding / drug effects
Toxoplasma / chemistry,  immunology
Grant Support
ID/Acronym/Agency:
AI-34760/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Antibodies; 56-65-5/Adenosine Triphosphate; EC 3.6.4.1/Myosins

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