Document Detail


Characterization of murine BATF: a negative regulator of activator protein-1 activity in the thymus.
MedLine Citation:
PMID:  11466704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BATF belongs to the AP-1/ATF superfamily of transcription factors and forms heterodimers with Jun proteins to bind AP-1 consensus DNA. Unlike Fos/Jun heterodimers which stimulate gene transcription, BATF/Jun heterodimers are transcriptionally inert and inhibit biological processes that are associated with the overstimulation of AP-1 activity. Here, we describe the murine BATF cDNA and genomic clones and map the BATF locus to chromosome 12 D2-3. Using in situ hybridization of BATF mRNA, we show that BATF gene expression is highly restricted, with the most prominent signals detected in the thymus. BATF mRNA levels are regulated differentially during discrete stages of T cell development and are up-regulated following activation of T cells in the periphery. To demonstrate the impact of BATF on AP-1 activity in vivo, AP-1 luciferase reporter mice were crossed to transgenic mice overexpressing BATF exclusively in thymic T cells. Results show that elevated levels of BATF protein correlate with reduced transactivation by AP-1. Since the differential regulation of AP-1 activity is linked to key transitions in the developing immune system, our observations support a critical role for BATF in determining the overall level of AP-1 activity, and thus AP-1 target gene expression, in specific T cell subtypes.
Authors:
K L Williams; I Nanda; G E Lyons; C T Kuo; M Schmid; J M Leiden; M H Kaplan; E J Taparowsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  European journal of immunology     Volume:  31     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-07-23     Completed Date:  2001-08-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1620-7     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
Data Bank Information
Bank Name/Acc. No.:
RefSeq/NM_016767
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Basic-Leucine Zipper Transcription Factors
Cell Differentiation
Cells, Cultured
Cloning, Molecular
Gene Expression Regulation, Developmental
Genes, Reporter / genetics
In Situ Hybridization
In Situ Hybridization, Fluorescence
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Transgenic
Molecular Sequence Data
Organ Specificity
Physical Chromosome Mapping
RNA, Messenger / genetics,  metabolism
Spleen / cytology,  immunology
T-Lymphocytes / cytology,  immunology,  metabolism
Thymus Gland / cytology,  immunology,  metabolism*
Transcription Factor AP-1 / antagonists & inhibitors*,  chemistry,  genetics,  metabolism*
Transcription Factors / chemistry,  genetics,  metabolism*
Transcriptional Activation
Grant Support
ID/Acronym/Agency:
CA-78264/CA/NCI NIH HHS; T32 (GM08298)/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Basic-Leucine Zipper Transcription Factors; 0/Batf protein, mouse; 0/RNA, Messenger; 0/Transcription Factor AP-1; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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