| Characterization of murine BATF: a negative regulator of activator protein-1 activity in the thymus. | |
MedLine Citation:
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PMID: 11466704 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BATF belongs to the AP-1/ATF superfamily of transcription factors and forms heterodimers with Jun proteins to bind AP-1 consensus DNA. Unlike Fos/Jun heterodimers which stimulate gene transcription, BATF/Jun heterodimers are transcriptionally inert and inhibit biological processes that are associated with the overstimulation of AP-1 activity. Here, we describe the murine BATF cDNA and genomic clones and map the BATF locus to chromosome 12 D2-3. Using in situ hybridization of BATF mRNA, we show that BATF gene expression is highly restricted, with the most prominent signals detected in the thymus. BATF mRNA levels are regulated differentially during discrete stages of T cell development and are up-regulated following activation of T cells in the periphery. To demonstrate the impact of BATF on AP-1 activity in vivo, AP-1 luciferase reporter mice were crossed to transgenic mice overexpressing BATF exclusively in thymic T cells. Results show that elevated levels of BATF protein correlate with reduced transactivation by AP-1. Since the differential regulation of AP-1 activity is linked to key transitions in the developing immune system, our observations support a critical role for BATF in determining the overall level of AP-1 activity, and thus AP-1 target gene expression, in specific T cell subtypes. |
Authors:
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K L Williams; I Nanda; G E Lyons; C T Kuo; M Schmid; J M Leiden; M H Kaplan; E J Taparowsky |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: European journal of immunology Volume: 31 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-07-23 Completed Date: 2001-08-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: Germany |
Other Details:
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Languages: eng Pagination: 1620-7 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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RefSeq/NM_016767 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Basic-Leucine Zipper Transcription Factors Cell Differentiation Cells, Cultured Cloning, Molecular Gene Expression Regulation, Developmental Genes, Reporter / genetics In Situ Hybridization In Situ Hybridization, Fluorescence Lymphocyte Activation Mice Mice, Inbred C57BL Mice, Transgenic Molecular Sequence Data Organ Specificity Physical Chromosome Mapping RNA, Messenger / genetics, metabolism Spleen / cytology, immunology T-Lymphocytes / cytology, immunology, metabolism Thymus Gland / cytology, immunology, metabolism* Transcription Factor AP-1 / antagonists & inhibitors*, chemistry, genetics, metabolism* Transcription Factors / chemistry, genetics, metabolism* Transcriptional Activation |
| Grant Support | |
ID/Acronym/Agency:
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CA-78264/CA/NCI NIH HHS; T32 (GM08298)/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Basic-Leucine Zipper Transcription Factors; 0/Batf protein, mouse; 0/RNA, Messenger; 0/Transcription Factor AP-1; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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