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Characterization of mouse cell line IMA 2.1 as a potential model system to study astrocyte functions.
MedLine Citation:
PMID:  22847254     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Astrocytes are activated in most chronic neurodegenerative diseases associated with inflammatory events such as Parkinson«s disease or Alzheimer«s disease, but also in stroke. Due to an aging population worldwide, research efforts in these areas are likely to expand in the future. This will entail an increased demand for appropriate experimental models. We introduce here the new immortalized mouse astrocyte cell line IMA 2.1 as an alternative to currently used primary astrocyte cultures. IMA 2.1 were directly compared with primary mouse astrocytes with respect to their response to proinflammatory stimuli, expression of typical astrocyte markers, and to the cell line«s capacity to metabolize the parkinsonian toxin MPTP to its toxic metabolite MPP+. Under inflammatory conditions, mimicked with the addition of a cytokine mix, IMA 2.1 responded similarly to primary astrocytes with mRNA upregulation, expression of iNOS and COX-2, and the release of various inflammatory mediators. Analysis of astrocytic markers indicated that IMA 2.1 represent a relatively early, GFAP-negative stage of astrocyte development. Moreover, conversion of MPTP by monoamine oxidase-B proceeded in IMA at least as quickly as in primary cells. For all endpoints investigated, the cell line IMA 2.1, derived from a single clone, delivered reproducible results over a period of several years and allowed upscaling of experiments due to its easy handling compared with primary cells.
Authors:
Stefan Schildknecht; Susanne Kirner; Anja Henn; Karlo Gasparic; Regina Pape; Liudmila Efremova; Olaf Maier; Roman Fischer; Marcel Leist
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  ALTEX     Volume:  29     ISSN:  1868-596X     ISO Abbreviation:  ALTEX     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100953980     Medline TA:  ALTEX     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  261-74     Citation Subset:  IM    
Affiliation:
Doerenkamp-Zbinden Chair for in vitro Toxicology and Biomedicine, University of Konstanz, Konstanz, Germany;
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