| Characterization of mononuclear phagocytic cells in medaka fish transgenic for a cxcr3a:gfp reporter. | |
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MedLine Citation:
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PMID: 20921403 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chemokines and chemokine receptors are key evolutionary innovations of vertebrates. They are involved in morphogenetic processes and play an important role in the immune system. Based on an analysis of the chemokine receptor gene family in teleost genomes, and the expression patterns of chemokine receptor genes during embryogenesis and the wounding response in young larvae of Oryzias latipes, we identified the chemokine receptor cxcr3a as a marker of innate immune cells. Cells expressing cxcr3a were characterized in fish transgenic for a cxcr3a:gfp reporter. In embryos and larvae, cxcr3a-expressing cells are motile in healthy and damaged tissues, and phagocytic; the majority of these cells has the morphology of tissue macrophages, whereas a small fraction has a dendritic phenotype. In adults, cxcr3a-positive cells continue to specifically express myeloid-associate markers and genes related to antigen uptake and presentation. By light microscopy and ultrastructural analysis, the majority of cxcr3a-expressing cells has a dendritic phenotype, whereas the remainder resembles macrophage-like cells. After challenge of adult fish with bacteria or CpG oligonucleotides, phagocytosing cxcr3a-positive cells in the blood up-regulated il12p40 genes, compatible with their function as part of the mononuclear phagocytic system. Our results identify a marker of teleost mononuclear phagocytic cells and suggest a surprising degree of morphological and functional similarity between the innate immune systems of lower and higher vertebrates. |
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Authors:
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Narges Aghaallaei; Baubak Bajoghli; Heinz Schwarz; Michael Schorpp; Thomas Boehm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-04 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-20 Completed Date: 2010-11-22 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 18079-84 Citation Subset: IM |
Affiliation:
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Department of Developmental Immunology, Max-Planck Institute of Immunobiology, D-79108 Freiburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Gene Expression Regulation, Developmental Genes, Reporter* Green Fluorescent Proteins / genetics Oryzias / embryology, genetics*, growth & development Phagocytes / cytology* Receptors, CXCR3 / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Receptors, CXCR3; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
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