Document Detail


Characterization of mononuclear cells remaining in the leukoreduction system chambers of apheresis instruments after routine platelet collection: a new source of viable human blood cells.
MedLine Citation:
PMID:  17524095     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The yield of white blood cells (WBCs) extracted from whole-blood leukoreduction filters can be affected by the storage conditions and delay before filtration. Platelets (PLTs) collected with apheresis instruments (Trima Accel, Gambro BCT) are leukoreduced during the procedure on a fluidized particle bed in a leukoreduction chamber (LRS chamber). In this report, the residual cell content of these LRS chambers was characterized to determine whether it would be a valuable source of viable human blood cells. STUDY DESIGN AND METHODS: The content of LRS chambers was eluted by gravity, and peripheral blood mononuclear cells (PBMNCs) were purified on a Ficoll-Paque gradient. Analyses were performed before and after freezing. Proportions of CD3+, CD14+, CD16+, CD19+, CD34+, and CD45+ cells were determined by flow cytometry. The frequency of T cells expressing CD4, CD8, and CD27 and of B cells expressing immunoglobulin G (IgG), IgM, and CD27 was also determined. RESULTS: LRS chambers held approximately 10(9) CD45+ cells representing the normal proportions of CD3+, CD14+, CD16+, and CD19+ cell populations of PBMNCs. A small fraction of these CD45+ cells were CD34+CD38+ cells (0.3 +/- 0.2%). The viability of these cells, measured before and after freezing, was more than 95 percent. CONCLUSION: The residual cell content of Trima Accel LRS chambers recovered after PLT collection is a good source of viable monocytes and lymphocytes. These PBMNCs, containing CD3+, CD14+, CD16+, CD19+, and CD34+ cells can be frozen to prepare cell banks, which opens new avenues for utilization in several physiologic studies or even in cellular therapy applications.
Authors:
Sonia Néron; Louis Thibault; Nathalie Dussault; Geneviève Côté; Eric Ducas; Nicolas Pineault; Annie Roy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  47     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-25     Completed Date:  2007-08-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1042-9     Citation Subset:  IM    
Affiliation:
Héma-Québec, Research and Development, Québec, Quebec, Canada. sonia.neron@hema-quebec.qc.ca
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis*
Cryopreservation
Humans
Leukocyte Reduction Procedures* / instrumentation
Lymphocytes / cytology*
Monocytes / cytology*
Plateletpheresis* / instrumentation
Tissue Therapy
Chemical
Reg. No./Substance:
0/Antigens, CD

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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