| Characterization of a monoclonal antibody ITI-Pl 1 directed against human platelet membrane glycoprotein IIb using extracts of whole platelets and platelet surface and intracellular membranes. | |
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MedLine Citation:
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PMID: 3278733 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A monoclonal antibody (mAb) termed ITI-Pl 1 has been prepared by the hybridoma procedure. Using immuno-absorption and crossed immunoelectrophoresis of Triton X-100 extracts of untreated and EDTA-treated human platelets it was shown to be directed against the surface membrane glycoprotein IIb (GP IIb). This mAb binds to whole platelets independently of ADP-stimulation and the presence of Ca2+-ions. It saturates at around 870 ng/10(8) cells corresponding to approximately 35,800 molecules/platelet. ITI-Pl 1 did not significantly inhibit GP IIb-IIIa dependent functions such as platelet aggregation or fibrinogen binding. Immunofluorescence could be demonstrated using ITI-Pl 1 and intact normal platelets, but not with platelets from a Glanzmann's thrombasthenia patient. Crossed immuno-electrophoresis with platelet extracts from four different thrombasthenic patients gave a line precipitate in the intermediate gel with 125I-labelled ITI-Pl 1 and autoradiography indicating trace amounts of free GP IIb or the GP IIb-IIIa complex. The epitope on GP IIb detected by ITI-Pl 1 is not destroyed by neuraminidase treatment. Thus the mAb also interacts with neuraminidase-treated GP IIb-IIIa complex in highly purified platelet surface membrane fractions as well as with GP IIb-IIIa from untreated internal membranes isolated by continuous flow electrophoresis. |
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Authors:
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L I Thorsen; G Gaudernack; N Hack; J M Wilkinson; F Brosstad; N O Solum; N Crawford |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of haematology Volume: 68 ISSN: 0007-1048 ISO Abbreviation: Br. J. Haematol. Publication Date: 1988 Jan |
Date Detail:
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Created Date: 1988-04-21 Completed Date: 1988-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372544 Medline TA: Br J Haematol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 67-74 Citation Subset: IM |
Affiliation:
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Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / isolation & purification* Blood Platelets / immunology* Cell Membrane / immunology Fluorescent Antibody Technique Humans Immunoelectrophoresis, Two-Dimensional Immunosorbent Techniques Intracellular Membranes / immunology Mice Platelet Membrane Glycoproteins / immunology* Thrombasthenia / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Platelet Membrane Glycoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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