Document Detail


Characterization of the molecular forms of glutathione S-transferase P1 in human gastric cancer cells (Kato III) and in normal human erythrocytes.
MedLine Citation:
PMID:  15471539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GSTP1 (glutathione S-transferase pi) is involved in stress responses and in cellular proliferation pathways as an inhibitor of JNK (c-Jun N-terminal kinase). It has been proposed that monomeric GSTP1 functions as a JNK inhibitor. All of the studies to date have been performed using rodent cells, and it is unclear if monomeric GSTP1 exists in human cells. Monomeric GSTP1 was sought in human gastric cancer cells (Kato III) and in normal human erythrocytes using gel filtration, ELISA and Western blots. Monomeric GSTP1 was found in conditioned medium, in cytosol of Kato III cells and in cytosol of erythrocytes. GSTP1 subunits from Kato III cells and erythrocytes were heterogeneous when analysed by MALDI-TOF (matrix-assisted laser-desorption ionization-time-of-flight) MS, suggesting that there were post-translational modifications to GSTP1. One post-translational modification, phosphorylation of a serine residue in the C-terminal portion of GSTP1 where JNK binds, was identified in GSTP1 purified from Kato III cells, but not in GSTP1 purified from human erythrocytes. Therefore normal and malignant human cells contain GSTP1 monomers with post-translational modifications, and it is likely that GSTP1 monomers regulate JNK activity in human cells in the same manner as in rodent cells.
Authors:
Perungavar N Ranganathan; Richard Whalen; Thomas D Boyer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  386     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-03     Completed Date:  2005-08-25     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  525-33     Citation Subset:  IM    
Affiliation:
The University of Arizona Liver Research Institute, 6309 AHSC, POB 245136, 1501 N. Campbell Ave., Tucson, AZ 85724-5136, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Line, Tumor
Chromatography, Gel
Culture Media, Conditioned / chemistry
Cytosol / enzymology
Dimerization
Enzyme-Linked Immunosorbent Assay
Erythrocytes / enzymology*
Glutathione S-Transferase pi
Glutathione Transferase / chemistry*,  isolation & purification,  metabolism*,  secretion
Humans
Isoenzymes / chemistry*,  isolation & purification,  metabolism*,  secretion
Mass Spectrometry
Phosphorylation
Protein Processing, Post-Translational
Protein Structure, Quaternary
Protein Subunits / chemistry,  isolation & purification,  metabolism,  secretion
Rodentia
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stomach Neoplasms / enzymology*
Grant Support
ID/Acronym/Agency:
ES06694/ES/NIEHS NIH HHS; GM31555/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Isoenzymes; 0/Protein Subunits; EC 2.5.1.18/GSTP1 protein, human; EC 2.5.1.18/Glutathione S-Transferase pi; EC 2.5.1.18/Glutathione Transferase
Comments/Corrections

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